| RRC ID |
45044
|
| 著者 |
Yasuda M, Hatanaka T, Shirato H, Nishioka T.
|
| タイトル |
Cell type-specific reciprocal regulation of HIF1A gene expression is dependent on 5'- and 3'-UTRs.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
In the present study, we demonstrated the reciprocal regulation of hypoxia-inducible factor 1 alpha (HIF1A) gene expression via untranslated region-(UTR) dependent mechanisms. A 151 nucleotide sequence found in the HIF1A 5'-UTR is sufficient for significant translational up-regulation. On the other hand, the 3'-UTR of HIF1A has been implicated in mRNA degradation. In the non-metastatic breast cancer cell line MCF7, the 3'-UTR-dependent down-regulatory machinery predominates over the 5'-UTR-dependent up-regulation of HIF1A. However, 5'-UTR-dependent up-regulation is dominant among metastatic cell lines (MDA-MB453, U87MG). It is therefore likely that the predominance of 5'-UTR-dependent translational enhancement of HIF1A is critical for the malignant phenotype of cancer cells. PTBP-1, but not HuR, is a candidate RNA binding protein for the translational control of HIF1A.
|
| 巻・号 |
447(4)
|
| ページ |
638-43
|
| 公開日 |
2014-5-16
|
| DOI |
10.1016/j.bbrc.2014.04.058
|
| PII |
S0006-291X(14)00700-1
|
| PMID |
24769203
|
| MeSH |
3' Untranslated Regions
5' Untranslated Regions
Base Sequence
Breast Neoplasms / genetics
Breast Neoplasms / metabolism
Cell Line, Tumor
Down-Regulation
Female
Gene Expression Regulation, Neoplastic
Glioma / genetics
Glioma / metabolism
HeLa Cells
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / genetics*
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
Luciferases / genetics
MCF-7 Cells
Molecular Sequence Data
Neoplasm Metastasis / genetics
RNA Processing, Post-Transcriptional
RNA Stability
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Neoplasm / genetics
RNA, Neoplasm / metabolism
Recombinant Proteins / genetics
Up-Regulation
|
| IF |
2.985
|
| 引用数 |
8
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa
MCF7(RCB1904)
MDA-MB-453(RCB1192)
U87MG |
