RRC ID 45066
Author Takei T, Kitazono J, Tanaka S, Nishimata H, Yoshida M.
Title Necrotic regions are absent in fiber-shaped cell aggregates, approximately 100 μm in diameter.
Journal Artif Cells Nanomed Biotechnol
Abstract Microscopic, fiber-shaped cell aggregates, have been used as building blocks for fabricating macroscopic three-dimensional tissue architectures, in the field of tissue engineering. In this study, we examined the occurrence of necrotic regions in the most widely used, fiber-shaped cell aggregates, approximately 100 μm in diameter. Alginate hydrogel hollow microfibers were used as templates for the cell aggregates. We demonstrated negligible necrotic region formation occurred in the cell aggregates formed in the hollow microfibers. Furthermore, we improved on previously-reported methods for preparing the hollow microfibers to avoid common microfiber tangling during the fiber preparation process.
Volume 44(1)
Pages 62-5
Published 2016
DOI 10.3109/21691401.2014.909824
PMID 24813225
MeSH Alginates / chemistry* Cell Aggregation Glucuronic Acid / chemistry Hep G2 Cells Hexuronic Acids / chemistry Humans Hydrogels / chemistry* Necrosis / prevention & control Tissue Engineering
IF 4.462
Times Cited 3
WOS Category ENGINEERING, BIOMEDICAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY MATERIALS SCIENCE, BIOMATERIALS
Resource
Human and Animal Cells