Reference - Detail
| RRC ID | 45183 |
|---|---|
| Author | Itoh T, Nakamura M, Nakamichi H, Ando M, Tsukamasa Y, Furuichi Y. |
| Title | Regulation of the differentiation of osteoblasts and osteoclasts by a hot-water extract of adzuki beans (Vigna angularis). |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Osteoporosis is a global public health problem thought to be caused by an imbalance in bone metabolism. We examined in this study the 40% ethanol fraction of HP-20 resin in combination with a hot-water adzuki extract (EtEx.40) for its effect on osteoblast and osteoclast differentiation. EtEx.40-treated murine preosteoblast MC3T3-E1 cells exhibited significantly elevated alkaline phosphatase activity and mineralization. EtEx.40 facilitated osteoblast differentiation by up-regulating such osteoblast differentiation-related molecules as runt-related transcription factor 2, distal-less homeobox 5, and osterix via p38 mitogen-activated protein kinase. EtEx.40 also suppressed the formation of large tartrate-resistant acid phosphatase-positive multinucleated cells in RAW264.7 cells that had been stimulated with the receptor activator of the nuclear factor κB ligand/macrophage colony-stimulating factor. EtEx.40 significantly inhibited NF-κB activation, thus reducing the expression of such downstream molecules as c-Fos and NFATc1. Our findings suggest that EtEx.40 could be used to maintain bone mass. |
| Volume | 78(1) |
| Pages | 92-9 |
| Published | 2014-1-1 |
| DOI | 10.1080/09168451.2014.877182 |
| PMID | 25036490 |
| MeSH | Animals Cell Differentiation / drug effects* Cell Line Fabaceae / chemistry* Hot Temperature* Macrophage Colony-Stimulating Factor / pharmacology Mice NF-kappa B / metabolism Osteoblasts / cytology* Osteoblasts / drug effects Osteoclasts / cytology* Osteoclasts / drug effects Plant Extracts / isolation & purification Plant Extracts / pharmacology* RANK Ligand / pharmacology Signal Transduction / drug effects Up-Regulation / drug effects Water / chemistry* p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 1.516 |
| Times Cited | 7 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |