RRC ID 45188
Author Okudela K, Suzuki T, Umeda S, Tateishi Y, Mitsui H, Miyagi Y, Ohashi K.
Title A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: potential involvement of miR-31 in lung carcinogenesis.
Journal Oncol Rep
Abstract Small non-protein coding RNAs that regulate messenger RNA levels, namely microRNAs (miRNAs), have been implicated in the pathogenesis of various diseases. The purpose of the present study was to identify essential miRNAs involved in lung carcinogenesis. Previous studies demonstrated that an investigation into the downstream targets of oncogenic KRAS could be used as a strategy to elucidate the molecular mechanisms involved in lung cancer; therefore, we examined the expression profiles of mRNAs modulated by oncogenic KRAS in the present study. We focused on miR-31 from the miRNAs that were differentially expressed, and evaluated its potential role in the development of lung cancer. miR-31 was upregulated not only by oncogenic KRAS, but also by oncogenic EGFR. The expression of miR-31 was markedly attenuated in some lung cancer cell lines by deleting its host gene locus. The restoration of miR-31 in lung cancer cell lines that lost its expression attenuated their growth activities. The knockdown of miR-31 expression in lung cancer cell lines retaining its expression enhanced anchorage-independent growth activity. These results suggest that miR-31 may be a suppressor that regulates an essential oncogenic pathway, the loss of which may promote lung carcinogenesis.
Volume 32(4)
Pages 1374-84
Published 2014-10-1
DOI 10.3892/or.2014.3339
PMID 25050641
MeSH Carcinogenesis / genetics* Carcinoma, Non-Small-Cell Lung / genetics* Cell Line Cell Line, Tumor ErbB Receptors / metabolism Gene Expression Regulation, Neoplastic / genetics* Humans Lung / cytology Lung / metabolism* Lung Neoplasms / genetics* MicroRNAs / genetics* Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins p21(ras) Small Cell Lung Carcinoma / genetics* Up-Regulation ras Proteins / metabolism*
IF 3.417
Times Cited 11
Human and Animal Cells LC-2/ad(RCB0440)