| RRC ID |
45188
|
| 著者 |
Okudela K, Suzuki T, Umeda S, Tateishi Y, Mitsui H, Miyagi Y, Ohashi K.
|
| タイトル |
A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: potential involvement of miR-31 in lung carcinogenesis.
|
| ジャーナル |
Oncol Rep
|
| Abstract |
Small non-protein coding RNAs that regulate messenger RNA levels, namely microRNAs (miRNAs), have been implicated in the pathogenesis of various diseases. The purpose of the present study was to identify essential miRNAs involved in lung carcinogenesis. Previous studies demonstrated that an investigation into the downstream targets of oncogenic KRAS could be used as a strategy to elucidate the molecular mechanisms involved in lung cancer; therefore, we examined the expression profiles of mRNAs modulated by oncogenic KRAS in the present study. We focused on miR-31 from the miRNAs that were differentially expressed, and evaluated its potential role in the development of lung cancer. miR-31 was upregulated not only by oncogenic KRAS, but also by oncogenic EGFR. The expression of miR-31 was markedly attenuated in some lung cancer cell lines by deleting its host gene locus. The restoration of miR-31 in lung cancer cell lines that lost its expression attenuated their growth activities. The knockdown of miR-31 expression in lung cancer cell lines retaining its expression enhanced anchorage-independent growth activity. These results suggest that miR-31 may be a suppressor that regulates an essential oncogenic pathway, the loss of which may promote lung carcinogenesis.
|
| 巻・号 |
32(4)
|
| ページ |
1374-84
|
| 公開日 |
2014-10-1
|
| DOI |
10.3892/or.2014.3339
|
| PMID |
25050641
|
| MeSH |
Carcinogenesis / genetics*
Carcinoma, Non-Small-Cell Lung / genetics*
Cell Line
Cell Line, Tumor
ErbB Receptors / metabolism
Gene Expression Regulation, Neoplastic / genetics*
Humans
Lung / cytology
Lung / metabolism*
Lung Neoplasms / genetics*
MicroRNAs / genetics*
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins p21(ras)
Small Cell Lung Carcinoma / genetics*
Up-Regulation
ras Proteins / metabolism*
|
| IF |
3.417
|
| 引用数 |
11
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
LC-2/ad(RCB0440) |
