論文 - 詳細
| RRC ID | 45244 |
|---|---|
| 著者 | Abdelwahab AF, Ohyama A, Higashi T, Motoyama K, Khaled KA, Sarhan HA, Hussein AK, Arima H. |
| タイトル | Preparation and evaluation of polyamidoamine dendrimer conjugate with glucuronylglucosyl-β-cyclodextrin (G3) as a novel carrier for siRNA. |
| ジャーナル | J Drug Target |
| Abstract |
Abstract In this study, we newly synthesized the polyamidoamine STARBURST dendrimer (dendrimer, generation 3: G3) conjugates with 6-O-α-(4-O-α-D-glucuronyl)-D-glucosyl-β-cyclodextrin [GUG-β-CDE (G3)] having the various degrees of substitution (DS) of GUG-β-cyclodextrin of 1.6, 3.0, 3.7, 5.0 and 8.6, and evaluated them as a siRNA transfer carrier. GUG-β-CDEs (G3) formed the positively charged and nano-order complexes with siRNA. Of the siRNA complexes with five GUG-β-CDEs (G3), the complex with GUG-β-CDE (G3, DS 3.7) showed the highest RNAi effect and cellular uptake with negligible cytotoxicity in KB cells at a charge ratio of 20. In addition, the RNAi effect and cellular uptake of the complex with GUG-β-CDE (G3, DS 3.7) were higher than those of α-CDE (G3, DS 2.4) and comparable to those of Lipofectamine™ 2000. Furthermore, the complex with GUG-β-CDE (G3, DS 3.7) possessed the endosomal escaping ability, the releasing property of siRNA in the cytoplasm and serum resistance. These results suggest that GUG-β-CDE (G3, DS 3.7) has the potential as a novel siRNA carrier. |
| 巻・号 | 22(10) |
| ページ | 927-34 |
| 公開日 | 2014-12-1 |
| DOI | 10.3109/1061186X.2014.950663 |
| PMID | 25148608 |
| MeSH | Dendrimers / chemistry* Endosomes / metabolism Gene Transfer Techniques Humans KB Cells Lipids / chemistry* Oligosaccharides / chemistry* RNA, Small Interfering / administration & dosage* |
| IF | 3.38 |
| 引用数 | 9 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Colon-26(RCB2657) |