論文 - 詳細
| RRC ID | 45257 |
|---|---|
| 著者 | Ishida S, Tanaka R, Yamaguchi N, Ogata G, Mizutani T, Kawabata K, Haga H. |
| タイトル | Epithelial sheet folding induces lumen formation by Madin-Darby canine kidney cells in a collagen gel. |
| ジャーナル | PLoS One |
| Abstract |
Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-β1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-β1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement. |
| 巻・号 | 9(8) |
| ページ | e99655 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0099655 |
| PII | PONE-D-14-00556 |
| PMID | 25170757 |
| PMC | PMC4149355 |
| MeSH | Animals Cell Movement* Collagen / chemistry* Dogs Epithelium / metabolism Extracellular Matrix / chemistry* Gels / chemistry Integrin beta Chains / metabolism Madin Darby Canine Kidney Cells Models, Biological* |
| IF | 2.74 |
| 引用数 | 9 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |