| RRC ID |
45257
|
| 著者 |
Ishida S, Tanaka R, Yamaguchi N, Ogata G, Mizutani T, Kawabata K, Haga H.
|
| タイトル |
Epithelial sheet folding induces lumen formation by Madin-Darby canine kidney cells in a collagen gel.
|
| ジャーナル |
PLoS One
|
| Abstract |
Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-β1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-β1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement.
|
| 巻・号 |
9(8)
|
| ページ |
e99655
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0099655
|
| PII |
PONE-D-14-00556
|
| PMID |
25170757
|
| PMC |
PMC4149355
|
| MeSH |
Animals
Cell Movement*
Collagen / chemistry*
Dogs
Epithelium / metabolism
Extracellular Matrix / chemistry*
Gels / chemistry
Integrin beta Chains / metabolism
Madin Darby Canine Kidney Cells
Models, Biological*
|
| IF |
2.74
|
| 引用数 |
9
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MDCK(RCB0995) |
