論文 - 詳細
| RRC ID | 45282 |
|---|---|
| 著者 | Sekine K, Kagawa Y, Maeyama E, Ota H, Haraguchi Y, Matsuura K, Shimizu T. |
| タイトル | Oxygen consumption of human heart cells in monolayer culture. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Tissue engineering in cardiovascular regenerative therapy requires the development of an efficient oxygen supply system for cell cultures. However, there are few studies which have examined human cardiomyocytes in terms of oxygen consumption and metabolism in culture. We developed an oxygen measurement system equipped with an oxygen microelectrode sensor and estimated the oxygen consumption rates (OCRs) by using the oxygen concentration profiles in culture medium. The heart is largely made up of cardiomyocytes, cardiac fibroblasts, and cardiac endothelial cells. Therefore, we measured the oxygen consumption of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs), cardiac fibroblasts, human cardiac microvascular endothelial cell and aortic smooth muscle cells. Then we made correlations with their metabolisms. In hiPSC-CMs, the value of the OCR was 0.71±0.38pmol/h/cell, whereas the glucose consumption rate and lactate production rate were 0.77±0.32pmol/h/cell and 1.61±0.70pmol/h/cell, respectively. These values differed significantly from those of the other cells in human heart. The metabolism of the cells that constitute human heart showed the molar ratio of lactate production to glucose consumption (L/G ratio) that ranged between 1.97 and 2.2. Although the energy metabolism in adult heart in vivo is reported to be aerobic, our data demonstrated a dominance of anaerobic glycolysis in an in vitro environment. With our measuring system, we clearly showed the differences in the metabolism of cells between in vivo and in vitro monolayer culture. Our results regarding cell OCRs and metabolism may be useful for future tissue engineering of human heart. |
| 巻・号 | 452(3) |
| ページ | 834-9 |
| 公開日 | 2014-9-26 |
| DOI | 10.1016/j.bbrc.2014.09.018 |
| PII | S0006-291X(14)01626-X |
| PMID | 25218502 |
| MeSH | Cell Differentiation Cells, Cultured Culture Media Endothelial Cells / cytology Endothelial Cells / metabolism* Glucose / metabolism Glycolysis / physiology Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Lactic Acid / biosynthesis Microelectrodes Myocytes, Cardiac / cytology Myocytes, Cardiac / metabolism* Myocytes, Smooth Muscle / cytology Myocytes, Smooth Muscle / metabolism* Myofibroblasts / cytology Myofibroblasts / metabolism* Oxygen / metabolism* Oxygen Consumption / physiology* Tissue Engineering |
| IF | 2.985 |
| 引用数 | 12 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |