論文 - 詳細
RRC ID | 45299 |
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著者 | Motoyama K, Mitsuyasu R, Akao C, Tanaka T, Ohyama A, Sato N, Higashi T, Arima H. |
タイトル | Design and evaluation of thioalkylated mannose-modified dendrimer (G3)/α-cyclodextrin conjugates as antigen-presenting cell-selective siRNA carriers. |
ジャーナル | AAPS J |
Abstract |
To design and evaluate the potential use of thioalkylated mannose-modified dendrimer (generation 3; G3) conjugates with α-cyclodextrin (Man-S-α-CDE (G3)) as novel antigen-presenting cell (APC)-selective siRNA carriers, we investigated the RNAi effects of siRNA complexes with Man-S-α-CDEs (G3). Man-S-α-CDE (G3, average degree of substitution of mannose (DSM) 4)/siRNA complex had the potent RNAi effects in both NR8383 cells, a rat alveolar macrophage cell line, and JAWSII cells, a mouse dendritic cell line, through adequate physicochemical properties, mannose receptor (MR)-mediated cellular uptake, and efficient phagosomal escape of the siRNA complex. In addition, cytotoxic activities of the siRNA complexes with α-CDE (G3, DS2) and Man-S-α-CDE (G3, DSM4) were almost negligible up to a charge ratio of 100 (carrier/siRNA). Taken together, these results suggest that Man-S-α-CDE (G3, DSM4) has the potential for a novel APC-selective siRNA carrier. |
巻・号 | 16(6) |
ページ | 1298-308 |
公開日 | 2014-11-1 |
DOI | 10.1208/s12248-014-9665-9 |
PMID | 25236864 |
PMC | PMC4389755 |
MeSH | Animals Antigen-Presenting Cells* / metabolism Cell Line Cell Survival / drug effects Dendrimers / chemistry* Dendritic Cells / drug effects Dendritic Cells / metabolism Drug Carriers / chemical synthesis Drug Carriers / chemistry* Drug Design* Gene Transfer Techniques Macrophages, Alveolar / drug effects Macrophages, Alveolar / metabolism Mannose / chemistry* Mice RNA Interference / drug effects RNA, Small Interfering / administration & dosage* RNA, Small Interfering / genetics Rats Transfection alpha-Cyclodextrins / chemistry* |
IF | 3.737 |
引用数 | 6 |
WOS 分野 | PHARMACOLOGY & PHARMACY |
リソース情報 | |
ヒト・動物細胞 | Colon-26(RCB2657) |