Reference - Detail
| RRC ID | 45341 |
|---|---|
| Author | Abiko Y, Mizokawa M, Kumagai Y. |
| Title | Activation of the Kelch-like ECH-associated protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2) pathway through covalent modification of the 2-alkenal group of aliphatic electrophiles in Coriandrum sativum L. |
| Journal | J Agric Food Chem |
| Abstract |
Phytochemicals able to activate the transcription factor NF-E2-related factor 2 (Nrf2) were isolated from an extract of Coriandrum sativum L. (C. sativum) leaves by preparative octadecyl silica column chromatography. Ultraperformance liquid chromatography and liquid chromatography-tandem mass spectrometry analysis of the isolated components after derivatization with 2-diphenylacetyl-1,3-inandione-1-hydrazone and experiments with HepG2 cells revealed that (E)-2-alkenals with different carbon numbers play a role in Nrf2 activation in these cells. Such Nrf2 activation appears to be attributable to S-alkylation of Kelch-like ECH-associated protein 1 (Keap1), the negative regulator for Nrf2, as determined by a biotin-PEAC5-maleimide assay. Interestingly, (E)-2-butenal caused Keap1 modification and Nrf2 activation, whereas butanal did not. These results suggest that (E)-2-alkenals with an α,β-unsaturated aldehyde moiety, which is a common substituent in phytochemicals isolated from C. sativum leaves, activate the Keap1/Nrf2 pathway associated with cellular protection. |
| Volume | 62(45) |
| Pages | 10936-44 |
| Published | 2014-11-12 |
| DOI | 10.1021/jf5030592 |
| PMID | 25307732 |
| MeSH | Alkenes / metabolism Alkylation / drug effects Coriandrum / chemistry* Hep G2 Cells Humans Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Kelch-Like ECH-Associated Protein 1 NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism* Plant Extracts / pharmacology* Signal Transduction / drug effects |
| IF | 4.192 |
| Times Cited | 8 |
| WOS Category | AGRICULTURE, MULTIDISCIPLINARY FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2 |