論文 - 詳細
| RRC ID | 45397 |
|---|---|
| 著者 | Nakamura I, Hama S, Itakura S, Takasaki I, Nishi T, Tabuchi Y, Kogure K. |
| タイトル | Lipocalin2 as a plasma marker for tumors with hypoxic regions. |
| ジャーナル | Sci Rep |
| Abstract |
Hypoxic tumors have been identified as appropriate indicators of tumor malignancy. However, no convenient plasma marker for hypoxic tumors has been described. Therefore, to identify a novel, convenient plasma marker for hypoxic tumors, we used microarray analysis to compare gene expression profiles of normoxic and hypoxic tumor tissues of mice bearing melanomas. Among the upregulated genes detected in hypoxic tumors, we chose to study the secretory protein lipocalin2 (LCN2) as a marker for hypoxic tumors. LCN2 protein levels in the plasma of mice bearing hypoxic tumors were significantly increased compared with those in mice bearing normoxic tumors. Interestingly, LCN2 mRNA levels were 17-fold higher in HIF-1α-positive hypoxic tumors than in HIF-1α-negative normoxic tumors. Furthermore, LCN2 mRNA levels were significantly higher in the B16-F1 cells and various human tumor cells cultured under hypoxic conditions than in cells cultured under normoxic conditions, while no changes in mRNA expression were observed in nontumor NIH-3T3 cells, even under hypoxic conditions. In cultured cells, the expression pattern of LCN2 was mostly consistent with that of HIF-1α, whereas that of a conventional hypoxic marker, carbonic anhydrase IX, was not. Collectively, our data suggested that LCN2 was a useful plasma marker for hypoxic tumors. |
| 巻・号 | 4 |
| ページ | 7235 |
| 公開日 | 2014-12-3 |
| DOI | 10.1038/srep07235 |
| PII | srep07235 |
| PMID | 25467539 |
| PMC | PMC4252902 |
| MeSH | Acute-Phase Proteins / metabolism Animals Biomarkers, Tumor / blood Biomarkers, Tumor / metabolism Cell Hypoxia / physiology Cell Line Cell Line, Tumor Hep G2 Cells Humans Hypoxia-Inducible Factor 1, alpha Subunit / analysis Lipocalin-2 Lipocalins / blood* Lipocalins / metabolism MCF-7 Cells Melanoma, Experimental / genetics Melanoma, Experimental / metabolism Mice NIH 3T3 Cells Oncogene Proteins / blood* Oncogene Proteins / metabolism Proto-Oncogene Proteins / metabolism RNA, Messenger / genetics Up-Regulation / genetics |
| IF | 3.998 |
| 引用数 | 6 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3 |