RRC ID 45401
Author Sugimoto K, Hayashi Y.
Title The Characteristics of Murine iPS Cells and siRNA Transfection Under Hypoxia.
Journal Methods Mol. Biol.
Abstract iPS cells are attractive for the regenerative medicine. The creation of pluripotent cells from somatic cells has great potential for basic and clinical research and application. Retroviral transduction of four or three transfection factors has been shown to initiate a reprogramming process. Here, we describe the effect of transcription factors regarding the growth and differentiation of mouse iPS cells in normoxia or hypoxia. Furthermore, we introduce the function of hypoxia-inducible factors (HIFs) in mouse iPS cells in hypoxia using RT-PCR and western blotting together with HIFs knockdown techniques.
Volume 1357
Pages 465-74
Published 2016
DOI 10.1007/7651_2014_158
PMID 25476444
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / antagonists & inhibitors Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / physiology* Blotting, Western / methods Cell Culture Techniques / methods* Cell Hypoxia Cells, Cultured Cellular Reprogramming* Cellular Reprogramming Techniques / methods* Gene Expression Regulation Hypoxia-Inducible Factor 1, alpha Subunit / antagonists & inhibitors Hypoxia-Inducible Factor 1, alpha Subunit / genetics Hypoxia-Inducible Factor 1, alpha Subunit / physiology* Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Mice Oxygen / pharmacology Oxygen / physiology RNA Interference* RNA, Small Interfering / genetics* Real-Time Polymerase Chain Reaction / methods Transcription Factors / antagonists & inhibitors Transcription Factors / biosynthesis Transcription Factors / genetics Transcription Factors / physiology* Transfection / methods*
Resource
Human and Animal Cells