RRC ID |
45417
|
著者 |
Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T.
|
タイトル |
Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation.
|
ジャーナル |
Biomacromolecules
|
Abstract |
Thermoresponsive, cationic, copolymer brushes poly(N-isopropylacrylamide(IPAAm)-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide(tBAAm)) and poly(IPAAm-co-3-acrylamidopropyl trimethylammonium chloride-co-tBAAm) were prepared on glass substrates through surface-initiated atom transfer radical polymerization. Prepared copolymer brushes were investigated as thermally modulated cell separation materials. Densely packed cationic copolymer brushes were formed on the glass substrates, and the positive charge density was modulated by controlling the composition of cationic moieties and species. During observation of cell adhesion and detachment properties on copolymer brushes, human bone marrow mesenchymal stem cells (hbmMSC) exhibited thermally modulated cell adhesion and detachment, while other bone-marrow-derived cells did not adhere. Using these properties, hbmMSC could be purified from mixtures of human bone-marrow-derived cells simply by changing the external temperature. Therefore, the prepared cationic copolymer brush is useful for separation of hbmMSC.
|
巻・号 |
16(2)
|
ページ |
532-40
|
公開日 |
2015-2-9
|
DOI |
10.1021/bm501591s
|
PMID |
25517012
|
MeSH |
Cell Adhesion / drug effects
Cell Adhesion / physiology
Cell Separation / methods*
Cells, Cultured
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / drug effects
Polymerization
Polymers / chemistry*
Polymers / pharmacology
Temperature*
|
IF |
6.092
|
引用数 |
33
|
WOS 分野
|
CHEMISTRY, ORGANIC
POLYMER SCIENCE
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
Human mesenchymal stem cells |