RRC ID 45417
Author Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T.
Title Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation.
Journal Biomacromolecules
Abstract Thermoresponsive, cationic, copolymer brushes poly(N-isopropylacrylamide(IPAAm)-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide(tBAAm)) and poly(IPAAm-co-3-acrylamidopropyl trimethylammonium chloride-co-tBAAm) were prepared on glass substrates through surface-initiated atom transfer radical polymerization. Prepared copolymer brushes were investigated as thermally modulated cell separation materials. Densely packed cationic copolymer brushes were formed on the glass substrates, and the positive charge density was modulated by controlling the composition of cationic moieties and species. During observation of cell adhesion and detachment properties on copolymer brushes, human bone marrow mesenchymal stem cells (hbmMSC) exhibited thermally modulated cell adhesion and detachment, while other bone-marrow-derived cells did not adhere. Using these properties, hbmMSC could be purified from mixtures of human bone-marrow-derived cells simply by changing the external temperature. Therefore, the prepared cationic copolymer brush is useful for separation of hbmMSC.
Volume 16(2)
Pages 532-40
Published 2015-2-9
DOI 10.1021/bm501591s
PMID 25517012
MeSH Cell Adhesion / drug effects Cell Adhesion / physiology Cell Separation / methods* Cells, Cultured Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects Polymerization Polymers / chemistry* Polymers / pharmacology Temperature*
IF 5.667
Times Cited 33
Human and Animal Cells