論文 - 詳細
| RRC ID | 45453 |
|---|---|
| 著者 | Katase N, Terada K, Suzuki T, Nishimatsu S, Nohno T. |
| タイトル | miR-487b, miR-3963 and miR-6412 delay myogenic differentiation in mouse myoblast-derived C2C12 cells. |
| ジャーナル | BMC Cell Biol |
| Abstract |
BACKGROUND:Skeletal muscle differentiation is a multistep, complex pathway in which several important signaling molecules are involved. Recently, microRNAs (miRNAs), endogenous non-coding small RNAs that regulate mRNAs, have been proposed to be involved in skeletal muscle differentiation. In this study, we identified skeletal muscle differentiation-associated miRNAs by comparing miRNA expression profiles between C2C12 cells and Wnt4 over-expressing C2C12 cells (W4-08), which can spontaneously differentiate into myotubes. RESULTS:We identified miR-206, miR-133a, and miR-133b as up-regulated miRNAs and miR-487b, miR-3963 and miR-6412 as down-regulated miRNAs in differentiating cells. We focused on the down-regulated miRNAs because their functions were largely unknown. Transfection of mimics of these miRNAs into C2C12 cells resulted in significantly reduced expression of myogenic differentiation markers, including troponin T and myosin heavy chain fast type and slow type, but did not affect the expression of the myogenic transcription factors, MyoD and myogenin. CONCLUSIONS:These miRNAs were characterized as new myogenic differentiation-associated miRNAs which may delay late myogenic differentiation or maturation. |
| 巻・号 | 16 |
| ページ | 13 |
| 公開日 | 2015-4-30 |
| DOI | 10.1186/s12860-015-0061-9 |
| PII | 10.1186/s12860-015-0061-9 |
| PMID | 25925429 |
| PMC | PMC4433089 |
| MeSH | Animals Cell Differentiation Cell Line Down-Regulation Mice MicroRNAs / antagonists & inhibitors MicroRNAs / genetics MicroRNAs / metabolism* MyoD Protein / metabolism Myoblasts / cytology Myoblasts / metabolism Myogenin / metabolism Myosin Heavy Chains / metabolism Oligonucleotides, Antisense / metabolism Transfection Troponin T / metabolism Up-Regulation Wnt4 Protein / genetics Wnt4 Protein / metabolism |
| IF | 3.066 |
| 引用数 | 12 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |