RRC ID 45481
Author Cieślar-Pobuda A, Jain MV, Kratz G, Rzeszowska-Wolny J, Ghavami S, Wiechec E.
Title The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.
Journal Oncotarget
Abstract Induced pluripotent stem cells (iPS) have become crucial in medicine and biology. Several studies indicate their phenotypic similarities with cancer stem cells (CSCs) and a propensity to form tumors. Thus it is desirable to identify a trait which differentiates iPS populations and CSCs. Searching for such a feature, in this work we compare the restriction (R) point-governed regulation of cell cycle progression in different cell types (iPS, cancer, CSC and normal cells) based on the expression profile of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1). Our study reveals that PFKFB3 and PFK1 expression allows discrimination between iPS and CSCs. Moreover, cancer and iPS cells, when cultured under hypoxic conditions, alter their expression level of PFKFB3 and PFK1 to resemble those in CSCs. We also observed cell type-related differences in response to inhibition of PFKFB3. This possibility to distinguish CSC from iPS cells or non-stem cancer cells by PFKB3 and PFK1 expression improves the outlook for clinical application of stem cell-based therapies and for more precise detection of CSCs.
Volume 6(30)
Pages 29753-70
Published 2015-10-6
DOI 10.18632/oncotarget.4995
PII 4995
PMID 26337471
PMC PMC4745760
MeSH Aldehyde Dehydrogenase / metabolism Cell Cycle / genetics Cell Line, Tumor Cells, Cultured Gene Expression Profiling* Humans Immunoblotting Induced Pluripotent Stem Cells / metabolism* Microscopy, Confocal Neoplasms / genetics Neoplasms / metabolism Neoplasms / pathology Neoplastic Stem Cells / metabolism* Phosphofructokinase-1 / genetics Phosphofructokinase-2 / genetics* Phosphofructokinase-2 / metabolism RNA Interference Reverse Transcriptase Polymerase Chain Reaction Spheroids, Cellular / metabolism
IF 5.168
Times Cited 30
Human and Animal Cells