論文 - 詳細
| RRC ID | 45500 |
|---|---|
| 著者 | Horie M, Warabi E, Komine S, Oh S, Shoda J. |
| タイトル | Cytoprotective Role of Nrf2 in Electrical Pulse Stimulated C2C12 Myotube. |
| ジャーナル | PLoS One |
| Abstract |
Regular physical exercise is central to a healthy lifestyle. However, exercise-related muscle contraction can induce reactive oxygen species and reactive nitrogen species (ROS/RNS) production in skeletal muscle. The nuclear factor-E2-related factor-2 (Nrf2) transcription factor is a cellular sensor for oxidative stress. Regulation of nuclear Nrf2 signaling regulates antioxidant responses and protects organ structure and function. However, the role of Nrf2 in exercise- or contraction-induced ROS/RNS production in skeletal muscle is not clear. In this study, using differentiated C2C12 cells and electrical pulse stimulation (EPS) of muscle contraction, we explored whether Nrf2 plays a role in the skeletal muscle response to muscle contraction-induced ROS/RNS. We found that EPS (40 V, 1 Hz, 2 ms) stimulated ROS/RNS accumulation and Nrf2 activation. We also showed that expression of NQO1, HO-1 and GCLM increased after EPS-induced muscle contraction and was remarkably suppressed in cells with Nrf2 knockdown. We also found that the antioxidant N-acetylcysteine (NAC) significantly attenuated Nrf2 activation after EPS, whereas the nitric oxide synthetase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME) did not. Furthermore, Nrf2 knockdown after EPS markedly decreased ROS/RNS redox potential and cell viability and increased expression of the apoptosis marker Annexin V in C2C12 myotubes. These results indicate that Nrf2 activation and expression of Nrf2 regulated-genes protected muscle against the increased ROS caused by EPS-induced muscle contraction. Thus, our findings suggest that Nrf2 may be a key factor for preservation of muscle function during muscle contraction. |
| 巻・号 | 10(12) |
| ページ | e0144835 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0144835 |
| PII | PONE-D-15-29461 |
| PMID | 26658309 |
| PMC | PMC4681703 |
| MeSH | Animals Antioxidants / metabolism Apoptosis / physiology Cell Line Cell Survival / physiology Cytoprotection Electric Stimulation Mice Muscle Fibers, Skeletal / metabolism Muscle, Skeletal / cytology Muscle, Skeletal / metabolism* NF-E2-Related Factor 2 / metabolism* Oxidative Stress / physiology Reactive Nitrogen Species / metabolism Reactive Oxygen Species / metabolism Signal Transduction |
| IF | 2.74 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |