論文 - 詳細
| RRC ID | 45559 |
|---|---|
| 著者 | Elewa A, Shirayama M, Kaymak E, Harrison PF, Powell DR, Du Z, Chute CD, Woolf H, Yi D, Ishidate T, Srinivasan J, Bao Z, Beilharz TH, Ryder SP, Mello CC. |
| タイトル | POS-1 Promotes Endo-mesoderm Development by Inhibiting the Cytoplasmic Polyadenylation of neg-1 mRNA. |
| ジャーナル | Dev Cell |
| Abstract |
The regulation of mRNA translation is of fundamental importance in biological mechanisms ranging from embryonic axis specification to the formation of long-term memory. POS-1 is one of several CCCH zinc-finger RNA-binding proteins that regulate cell fate specification during C. elegans embryogenesis. Paradoxically, pos-1 mutants exhibit striking defects in endo-mesoderm development but have wild-type distributions of SKN-1, a key determinant of endo-mesoderm fates. RNAi screens for pos-1 suppressors identified genes encoding the cytoplasmic poly(A)-polymerase homolog GLD-2, the Bicaudal-C homolog GLD-3, and the protein NEG-1. We show that NEG-1 localizes in anterior nuclei, where it negatively regulates endo-mesoderm fates. In posterior cells, POS-1 binds the neg-1 3' UTR to oppose GLD-2 and GLD-3 activities that promote NEG-1 expression and cytoplasmic lengthening of the neg-1 mRNA poly(A) tail. Our findings uncover an intricate series of post-transcriptional regulatory interactions that, together, achieve precise spatial expression of endo-mesoderm fates in C. elegans embryos. |
| 巻・号 | 34(1) |
| ページ | 108-18 |
| 公開日 | 2015-7-6 |
| DOI | 10.1016/j.devcel.2015.05.024 |
| PII | S1534-5807(15)00360-3 |
| PMID | 26096734 |
| PMC | PMC4507413 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism* Cell Differentiation / physiology Cytoplasm / metabolism* Gene Expression Regulation, Developmental / physiology Germ Cells / metabolism Mesoderm / metabolism Nuclear Proteins / metabolism* Polyadenylation / physiology* RNA, Helminth / genetics RNA, Helminth / metabolism* RNA, Messenger / metabolism* RNA-Binding Proteins |
| IF | 10.092 |
| 引用数 | 5 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6077 |