RRC ID 45569
Author Govindan JA, Jayamani E, Zhang X, Mylonakis E, Ruvkun G.
Title Dialogue between E. coli free radical pathways and the mitochondria of C. elegans.
Journal Proc Natl Acad Sci U S A
Abstract The microbial world presents a complex palette of opportunities and dangers to animals, which have developed surveillance and response strategies to hints of microbial intent. We show here that the mitochondrial homeostatic response pathway of the nematode Caenorhabditis elegans responds to Escherichia coli mutations that activate free radical detoxification pathways. Activation of C. elegans mitochondrial responses could be suppressed by additional mutations in E. coli, suggesting that C. elegans responds to products of E. coli to anticipate challenges to its mitochondrion. Out of 50 C. elegans gene inactivations known to mediate mitochondrial defense, we found that 7 genes were required for C. elegans response to a free radical producing E. coli mutant, including the bZip transcription factor atfs-1 (activating transcription factor associated with stress). An atfs-1 loss-of-function mutant was partially resistant to the effects of free radical-producing E. coli mutant, but a constitutively active atfs-1 mutant growing on wild-type E. coli inappropriately activated the pattern of mitochondrial responses normally induced by an E. coli free radical pathway mutant. Carbonylated proteins from free radical-producing E. coli mutant may directly activate the ATFS-1/bZIP transcription factor to induce mitochondrial stress response: feeding C. elegans with H2O2-treated E. coli induces the mitochondrial unfolded protein response, and inhibition of a gut peptide transporter partially suppressed C. elegans response to free radical damaged E. coli.
Volume 112(40)
Pages 12456-61
Published 2015-10-6
DOI 10.1073/pnas.1517448112
PII 1517448112
PMID 26392561
PMC PMC4603447
MeSH Animals Animals, Genetically Modified Biosynthetic Pathways / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli / physiology Free Radicals / metabolism* Gene Expression Host-Pathogen Interactions / genetics Microscopy, Fluorescence Mitochondria / metabolism* Mutation Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / genetics Transcription Factors / metabolism
IF 9.412
Times Cited 17
C.elegans tm4525