| RRC ID |
45604
|
| Author |
Gengyo-Ando K, Mitani S.
|
| Title |
Characterization of mutations induced by ethyl methanesulfonate, UV, and trimethylpsoralen in the nematode Caenorhabditis elegans.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The genome project of the nematode Caenorhabditis elegans is completed. It is important and useful to disrupt nematode genes to know their function. We treated wild-type animals with potential candidates for mutagens for reverse genetics, EMS (ethyl methanesulfonate), short-wavelength UV, and long-wavelength UV in the presence of TMP (trimethylpsoralen). We estimated forward mutation rates by counting the occurrence of a marker unc-22 mutation. We found that the forward mutation rate by TMP/UV could be comparable with EMS by improving the frequency one order higher than before. We next isolated mutants of another marker gene ben-1 and examined the probability for the deletion mutations by PCR and sequencing. Deletion mutations were found only by TMP/UV method, which suggested TMP/UV is the choice for deletion mutagenesis among these methods. As a pilot experiment, we could isolate actual deletion mutations at a much higher frequency than previously.
|
| Volume |
269(1)
|
| Pages |
64-9
|
| Published |
2000-3-5
|
| DOI |
10.1006/bbrc.2000.2260
|
| PII |
S0006-291X(00)92260-5
|
| PMID |
10694478
|
| MeSH |
Animals
Base Sequence
Caenorhabditis elegans / drug effects
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / radiation effects
DNA Primers / genetics
DNA, Helminth / genetics
Ethyl Methanesulfonate / toxicity
Genes, Helminth / drug effects
Genes, Helminth / radiation effects
Genetic Markers
Mutation*
Phenotype
Sequence Deletion
Trioxsalen / toxicity
Ultraviolet Rays / adverse effects
|
| IF |
2.985
|
| Times Cited |
152
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| C.elegans |
tm25
tm124
tm177
tm234 |
