論文 - 詳細
| RRC ID | 45625 |
|---|---|
| 著者 | Phillips CM, Brown KC, Montgomery BE, Ruvkun G, Montgomery TA. |
| タイトル | piRNAs and piRNA-Dependent siRNAs Protect Conserved and Essential C. elegans Genes from Misrouting into the RNAi Pathway. |
| ジャーナル | Dev Cell |
| Abstract |
piRNAs silence foreign genes, such as transposons, to preserve genome integrity, but they also target endogenous mRNAs by mechanisms that are poorly understood. Caenorhabditis elegans piRNAs interact with both transposon and nontransposon mRNAs to initiate sustained silencing via the RNAi pathway. To assess the dysregulation of gene silencing caused by lack of piRNAs, we restored RNA silencing in RNAi-defective animals in the presence or absence of piRNAs. In the absence of piRNAs and a cellular memory of piRNA activity, essential and conserved genes are misrouted into the RNAi pathway to produce siRNAs that bind the nuclear Argonaute HRDE-1, resulting in dramatic defects in germ cell proliferation and function such that the animals are sterile. Inactivation of RNAi suppresses sterility, indicating that aberrant siRNAs produced in the absence of piRNAs target essential genes for silencing. Thus, by reanimating RNAi, we uncovered a role for piRNAs in protecting essential genes from RNA silencing. |
| 巻・号 | 34(4) |
| ページ | 457-65 |
| 公開日 | 2015-8-24 |
| DOI | 10.1016/j.devcel.2015.07.009 |
| PII | S1534-5807(15)00460-8 |
| PMID | 26279487 |
| PMC | PMC4550515 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Conserved Sequence* Female Fertility Genes, Essential* Genes, Helminth* High-Throughput Nucleotide Sequencing Molecular Sequence Data Protein Binding RNA Interference* RNA, Small Interfering / metabolism* |
| IF | 10.092 |
| 引用数 | 30 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1301 tm1358 tm1155 |