Reference - Detail
RRC ID | 45673 |
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Author | Ailion M, Hannemann M, Dalton S, Pappas A, Watanabe S, Hegermann J, Liu Q, Han HF, Gu M, Goulding MQ, Sasidharan N, Schuske K, Hullett P, Eimer S, Jorgensen EM. |
Title | Two Rab2 interactors regulate dense-core vesicle maturation. |
Journal | Neuron |
Abstract |
Peptide neuromodulators are released from a unique organelle: the dense-core vesicle. Dense-core vesicles are generated at the trans-Golgi and then sort cargo during maturation before being secreted. To identify proteins that act in this pathway, we performed a genetic screen in Caenorhabditis elegans for mutants defective in dense-core vesicle function. We identified two conserved Rab2-binding proteins: RUND-1, a RUN domain protein, and CCCP-1, a coiled-coil protein. RUND-1 and CCCP-1 colocalize with RAB-2 at the Golgi, and rab-2, rund-1, and cccp-1 mutants have similar defects in sorting soluble and transmembrane dense-core vesicle cargos. RUND-1 also interacts with the Rab2 GAP protein TBC-8 and the BAR domain protein RIC-19, a RAB-2 effector. In summary, a pathway of conserved proteins controls the maturation of dense-core vesicles at the trans-Golgi network. |
Volume | 82(1) |
Pages | 167-80 |
Published | 2014-4-2 |
DOI | 10.1016/j.neuron.2014.02.017 |
PII | S0896-6273(14)00115-9 |
PMID | 24698274 |
PMC | PMC3997996 |
MeSH | Analysis of Variance Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Mapping Cloning, Molecular Endosomes / metabolism Endosomes / ultrastructure Golgi Apparatus / metabolism Golgi Apparatus / ultrastructure Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Electron, Transmission Molecular Sequence Data Mutation / genetics* Protein Transport Secretory Vesicles / genetics Secretory Vesicles / metabolism* Secretory Vesicles / ultrastructure rab2 GTP-Binding Protein / genetics rab2 GTP-Binding Protein / metabolism* |
IF | 14.415 |
Times Cited | 30 |
WOS Category | NEUROSCIENCES |
Resource | |
C.elegans | tm3622 |