RRC ID 457
Author Matsunaga TM, Fujiwara H.
Title Identification and characterization of genes abnormally expressed in wing-deficient mutant (flügellos) of the silkworm, Bombyx mori.
Journal Insect Biochem. Mol. Biol.
Abstract The wing-deficient mutant, flügellos (fl), of the silkworm lacks four wings in the pupa and the adult, due to aberrant wing morphogenesis during metamorphosis. To elucidate the mechanisms of wing-specific deficiencies in the fl mutant, we used mRNA differential display and identified five genes abnormally expressed in the fl wing discs. Northern blot and RT-PCR analyses revealed that four genes were overexpressed, but the fifth one was not transcribed in the fl wing discs. The expression level of ribosome-associated protein p40 in the fl wing discs was elevated approximately 10 times compared to the wild-type (WT) discs. Another overexpressed gene CB10 encodes a novel wing-specific protein with a putative zinc-finger motif. Overexpression of two components of extracellular matrix, cuticle protein 18 (BMCP18) and a fibrillin-like protein AD10, may result in the abnormal wing morphogenesis in the fl mutant. In contrast, a novel member of multifunctional Ca2+-binding protein annexins, designated as annexin b13 (Anx b13), was expressed dominantly in the wing discs of WT but completely repressed in the fl tissues. Strong expression of Anx b13 in wing discs during the fourth and fifth instar indicates that ANX B13 plays an important role in wing morphogenesis.
Volume 32(6)
Pages 691-9
Published 2002-6
PII S0965174801001485
PMID 12020843
MeSH Amino Acid Sequence Animals Bombyx / anatomy & histology Bombyx / genetics* Extracellular Matrix Proteins / genetics Fibrillins Gene Expression* Gene Expression Profiling Insect Proteins / genetics* Microfilament Proteins / genetics Molecular Sequence Data RNA, Messenger Ribosomal Proteins / genetics Wings, Animal Zinc Fingers
IF 3.618
Times Cited 13
Silkworms flk strain