| RRC ID |
45701
|
| Author |
Cecere G, Hoersch S, O'Keeffe S, Sachidanandam R, Grishok A.
|
| Title |
Global effects of the CSR-1 RNA interference pathway on the transcriptional landscape.
|
| Journal |
Nat Struct Mol Biol
|
| Abstract |
Argonaute proteins and their small RNA cofactors short interfering RNAs are known to inhibit gene expression at the transcriptional and post-transcriptional levels. In Caenorhabditis elegans, the Argonaute CSR-1 binds thousands of endogenous siRNAs (endo-siRNAs) that are antisense to germline transcripts. However, its role in gene expression regulation remains controversial. Here we used genome-wide profiling of nascent RNA transcripts and found that the CSR-1 RNA interference pathway promoted sense-oriented RNA polymerase II transcription. Moreover, a loss of CSR-1 function resulted in global increase in antisense transcription and ectopic transcription of silent chromatin domains, which led to reduced chromatin incorporation of centromere-specific histone H3. On the basis of these findings, we propose that the CSR-1 pathway helps maintain the directionality of active transcription, thereby propagating the distinction between transcriptionally active and silent genomic regions.
|
| Volume |
21(4)
|
| Pages |
358-65
|
| Published |
2014-4-1
|
| DOI |
10.1038/nsmb.2801
|
| PII |
nsmb.2801
|
| PMID |
24681887
|
| PMC |
PMC4068146
|
| MeSH |
Animals
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / metabolism
Caenorhabditis elegans Proteins / physiology*
Chromatin / metabolism
Chromatin Assembly and Disassembly
Gene Expression Regulation
Histones / metabolism
RNA Interference*
RNA, Small Interfering
Transcription, Genetic*
|
| IF |
11.98
|
| Times Cited |
30
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm892 |
