論文 - 詳細
| RRC ID | 45711 |
|---|---|
| 著者 | Chuang M, Goncharov A, Wang S, Oegema K, Jin Y, Chisholm AD. |
| タイトル | The microtubule minus-end-binding protein patronin/PTRN-1 is required for axon regeneration in C. elegans. |
| ジャーナル | Cell Rep |
| Abstract |
Precise regulation of microtubule (MT) dynamics is increasingly recognized as a critical determinant of axon regeneration. In contrast to developing neurons, mature axons exhibit noncentrosomal microtubule nucleation. The factors regulating noncentrosomal MT architecture in axon regeneration remain poorly understood. We report that PTRN-1, the C. elegans member of the Patronin/Nezha/calmodulin- and spectrin-associated protein (CAMSAP) family of microtubule minus-end-binding proteins, is critical for efficient axon regeneration in vivo. ptrn-1-null mutants display generally normal developmental axon outgrowth but significantly impaired regenerative regrowth after laser axotomy. Unexpectedly, mature axons in ptrn-1 mutants display elevated numbers of dynamic axonal MTs before and after injury, suggesting that PTRN-1 inhibits MT dynamics. The CKK domain of PTRN-1 is necessary and sufficient for its functions in axon regeneration and MT dynamics and appears to stabilize MTs independent of minus-end localization. Whereas in developing neurons, PTRN-1 inhibits activity of the DLK-1 mitogen-activated protein kinase (MAPK) cascade, we find that, in regeneration, PTRN-1 and DLK-1 function together to promote axonal regrowth. |
| 巻・号 | 9(3) |
| ページ | 874-83 |
| 公開日 | 2014-11-6 |
| DOI | 10.1016/j.celrep.2014.09.054 |
| PII | S2211-1247(14)00837-7 |
| PMID | 25437544 |
| PMC | PMC4250830 |
| MeSH | Animals Axons / metabolism* Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Microtubule-Associated Proteins / chemistry Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Motor Neurons / cytology Motor Neurons / metabolism Mutation Polymerization Protein Binding Protein Structure, Tertiary Regeneration* Sensory Receptor Cells / cytology Sensory Receptor Cells / metabolism Structure-Activity Relationship Up-Regulation |
| IF | 8.109 |
| 引用数 | 36 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5597 tm4024 tm2143 tm2807 |