RRC ID 45759
著者 Jobson MA, Valdez CM, Gardner J, Garcia LR, Jorgensen EM, Beg AA.
タイトル Spillover transmission is mediated by the excitatory GABA receptor LGC-35 in C. elegans.
ジャーナル J Neurosci
Abstract Under most circumstances, GABA activates chloride-selective channels and thereby inhibits neuronal activity. Here, we identify a GABA receptor in the nematode Caenorhabditis elegans that conducts cations and is therefore excitatory. Expression in Xenopus oocytes demonstrates that LGC-35 is a homopentameric cation-selective receptor of the cys-loop family exclusively activated by GABA. Phylogenetic analysis suggests that LGC-35 evolved from GABA-A receptors, but the pore-forming domain contains novel molecular determinants that confer cation selectivity. LGC-35 is expressed in muscles and directly mediates sphincter muscle contraction in the defecation cycle in hermaphrodites, and spicule eversion during mating in the male. In the locomotory circuit, GABA release directly activates chloride channels on the muscle to cause muscle relaxation. However, GABA spillover at these synapses activates LGC-35 on acetylcholine motor neurons, which in turn cause muscles to contract, presumably to drive wave propagation along the body. These studies demonstrate that both direct and indirect excitatory GABA signaling plays important roles in regulating neuronal circuit function and behavior in C. elegans.
巻・号 35(6)
ページ 2803-16
公開日 2015-2-11
DOI 10.1523/JNEUROSCI.4557-14.2015
PII 35/6/2803
PMID 25673867
PMC PMC4323542
MeSH Acetylcholine / physiology Animals Behavior, Animal Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Copulation / physiology Defecation Male Motor Neurons / physiology Muscle Contraction / physiology Muscles / physiology* Oocytes / drug effects Receptors, GABA / genetics Receptors, GABA / physiology* Sexual Behavior, Animal / physiology Synaptic Transmission / physiology* Xenopus laevis
IF 5.674
引用数 11
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm1444