| RRC ID |
45764
|
| Author |
Kasper DM, Wang G, Gardner KE, Johnstone TG, Reinke V.
|
| Title |
The C. elegans SNAPc component SNPC-4 coats piRNA domains and is globally required for piRNA abundance.
|
| Journal |
Dev Cell
|
| Abstract |
The Piwi/Piwi-interacting RNA (piRNA) pathway protects the germline from the activity of foreign sequences such as transposons. Remarkably, tens of thousands of piRNAs arise from a minimal number of discrete genomic regions. The extent to which clustering of these small RNA genes contributes to their coordinated expression remains unclear. We show that C. elegans SNPC-4, the Myb-like DNA-binding subunit of the small nuclear RNA activating protein complex, binds piRNA clusters in a germline-specific manner and is required for global piRNA expression. SNPC-4 localization is mutually dependent with localization of piRNA biogenesis factor PRDE-1. SNPC-4 exhibits an atypical widely distributed binding pattern that "coats" piRNA domains. Discrete peaks within the domains occur frequently at RNA-polymerase-III-occupied transfer RNA (tRNA) genes, which have been implicated in chromatin organization. We suggest that SNPC-4 binding establishes a positive expression environment across piRNA domains, providing an explanation for the conserved clustering of individually transcribed piRNA genes.
|
| Volume |
31(2)
|
| Pages |
145-58
|
| Published |
2014-10-27
|
| DOI |
10.1016/j.devcel.2014.09.015
|
| PII |
S1534-5807(14)00621-2
|
| PMID |
25373775
|
| PMC |
PMC4223638
|
| MeSH |
Animals
Base Sequence
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Cell Nucleus / metabolism
Chromatin / genetics
Chromosomal Proteins, Non-Histone / genetics
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Epigenesis, Genetic
RNA Polymerase III / genetics
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism*
Sequence Analysis, RNA
Signal Transduction / genetics
Transcription Factors / genetics
|
| IF |
10.092
|
| Times Cited |
15
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm4568
tm1813. tm2303
tm0872 |
