論文 - 詳細
| RRC ID | 45779 |
|---|---|
| 著者 | LaBonty M, Szmygiel C, Byrnes LE, Hughes S, Woollard A, Cram EJ. |
| タイトル | CACN-1/Cactin plays a role in Wnt signaling in C. elegans. |
| ジャーナル | PLoS One |
| Abstract |
Wnt signaling is tightly regulated during animal development and controls cell proliferation and differentiation. In C. elegans, activation of Wnt signaling alters the activity of the TCF/LEF transcription factor, POP-1, through activation of the Wnt/β-catenin or Wnt/β-catenin asymmetry pathways. In this study, we have identified CACN-1 as a potential regulator of POP-1 in C. elegans larval development. CACN-1/Cactin is a well-conserved protein of unknown molecular function previously implicated in the regulation of several developmental signaling pathways. Here we have used activation of POPTOP, a POP-1-responsive reporter construct, as a proxy for Wnt signaling. POPTOP requires POP-1 and SYS-1/β-catenin for activation in L4 uterine cells. RNAi depletion experiments show that CACN-1 is needed to prevent excessive activation of POPTOP and for proper levels and/or localization of POP-1. Surprisingly, high POPTOP expression correlates with increased levels of POP-1 in uterine nuclei, suggesting POPTOP may not mirror endogenous gene expression in all respects. Genetic interaction studies suggest that CACN-1 may act partially through LIT-1/NLK to alter POP-1 localization and POPTOP activation. Additionally, CACN-1 is required for proper proliferation of larval seam cells. Depletion of CACN-1 results in a loss of POP-1 asymmetry and reduction of terminal seam cell number, suggesting an adoption of the anterior, differentiated fate by the posterior daughter cells. These findings suggest CACN-1/Cactin modulates Wnt signaling during larval development. |
| 巻・号 | 9(7) |
| ページ | e101945 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0101945 |
| PII | PONE-D-13-14081 |
| PMID | 24999833 |
| PMC | PMC4084952 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Division Cell Proliferation DNA-Binding Proteins / metabolism Gene Expression Regulation, Developmental High Mobility Group Proteins / metabolism Larva / cytology Larva / metabolism Male Membrane Proteins / metabolism Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Serine-Threonine Kinases / metabolism Protein Transport RNA Interference Testis / cytology Testis / growth & development Transcription, Genetic Wnt Signaling Pathway* beta Catenin / metabolism |
| IF | 2.74 |
| 引用数 | 10 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3042 |