| RRC ID |
45802
|
| Author |
Murakami-Tonami Y, Ikeda H, Yamagishi R, Inayoshi M, Inagaki S, Kishida S, Komata Y, Jan Koster, Takeuchi I, Kondo Y, Maeda T, Sekido Y, Murakami H, Kadomatsu K.
|
| Title |
SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells.
|
| Journal |
Sci Rep
|
| Abstract |
Shugoshin 1 (SGO1) is required for accurate chromosome segregation during mitosis and meiosis; however, its other functions, especially at interphase, are not clearly understood. Here, we found that downregulation of SGO1 caused a synergistic phenotype in cells overexpressing MYCN. Downregulation of SGO1 impaired proliferation and induced DNA damage followed by a senescence-like phenotype only in MYCN-overexpressing neuroblastoma cells. In these cells, SGO1 knockdown induced DNA damage, even during interphase, and this effect was independent of cohesin. Furthermore, MYCN-promoted SGO1 transcription and SGO1 expression tended to be higher in MYCN- or MYC-overexpressing cancers. Together, these findings indicate that SGO1 plays a role in the DNA damage response in interphase. Therefore, we propose that SGO1 represents a potential molecular target for treatment of MYCN-amplified neuroblastoma.
|
| Volume |
6
|
| Pages |
31615
|
| Published |
2016-8-19
|
| DOI |
10.1038/srep31615
|
| PII |
srep31615
|
| PMID |
27539729
|
| PMC |
PMC4990925
|
| MeSH |
Animals
Cell Cycle Proteins / biosynthesis*
Cell Cycle Proteins / genetics
Cell Line, Tumor
DNA Damage*
Gene Amplification*
Gene Expression Regulation, Neoplastic*
Interphase / genetics
Mice
Mice, Transgenic
N-Myc Proto-Oncogene Protein / genetics
N-Myc Proto-Oncogene Protein / metabolism*
Neuroblastoma / genetics
Neuroblastoma / metabolism*
Neuroblastoma / pathology
Transcription, Genetic*
|
| IF |
3.998
|
| Times Cited |
6
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| DNA material |
CSII-CMV-RfA-IRES2-Venus (RDB04388)
CSII-CMV-Venus (RDB05553)
CSII-CMV-mRFP (RDB06386). |
