RRC ID 45810
Author Morrissey MA, Keeley DP, Hagedorn EJ, McClatchey STH, Chi Q, Hall DH, Sherwood DR.
Title B-LINK: a hemicentin, plakin, and integrin-dependent adhesion system that links tissues by connecting adjacent basement membranes.
Journal Dev Cell
Abstract Basement membrane (BM), a sheet-like form of extracellular matrix, surrounds most tissues. During organogenesis, specific adhesions between adjoining tissues frequently occur; however, their molecular basis is unclear. Using live-cell imaging and electron microscopy, we identify an adhesion system that connects the uterine and gonadal tissues through their juxtaposed BMs at the site of anchor cell (AC) invasion in C. elegans. We find that the extracellular matrix component hemicentin (HIM-4), found between BMs, forms punctate accumulations under the AC and controls BM linkage to promote rapid invasion. Through targeted screening, we identify the integrin-binding cytolinker plakin (VAB-10A) and integrin (INA-1/PAT-3) as key BM-BM linkage regulators: VAB-10A localizes to the AC-BM interface and tethers hemicentin to the AC while integrin promotes hemicentin punctae formation. Together, plakin, integrin, and hemicentin are founding components of a cell-directed adhesion system, which we name a BM-LINKage (B-LINK), that connects adjacent tissues through adjoining BMs.
Volume 31(3)
Pages 319-331
Published 2014-11-10
DOI 10.1016/j.devcel.2014.08.024
PII S1534-5807(14)00554-1
PMID 25443298
PMC PMC4254419
MeSH Animals Basement Membrane / metabolism* Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Adhesion / physiology Cell Movement Extracellular Matrix / metabolism Integrin beta Chains / metabolism* Membrane Proteins / metabolism*
IF 9.19
Times Cited 31
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
C.elegans tm1962