| RRC ID |
45812
|
| 著者 |
Nagy AI, Vázquez-Manrique RP, Lopez M, Christov CP, Sequedo MD, Herzog M, Herlihy AE, Bodak M, Gatsi R, Baylis HA.
|
| タイトル |
IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans.
|
| ジャーナル |
EMBO Rep
|
| Abstract |
RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment.
|
| 巻・号 |
16(3)
|
| ページ |
341-50
|
| 公開日 |
2015-3-1
|
| DOI |
10.15252/embr.201439585
|
| PII |
embr.201439585
|
| PMID |
25608529
|
| PMC |
PMC4353841
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology*
Image Processing, Computer-Assisted
Inositol 1,4,5-Trisphosphate / metabolism*
Intestinal Mucosa / metabolism
Microscopy, Fluorescence
Models, Biological
RNA Interference / physiology*
RNA, Double-Stranded
Signal Transduction / genetics
Signal Transduction / physiology*
|
| IF |
7.497
|
| 引用数 |
1
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm902
tm753 |
