RRC ID 45817
Author Ni JZ, Chen E, Gu SG.
Title Complex coding of endogenous siRNA, transcriptional silencing and H3K9 methylation on native targets of germline nuclear RNAi in C. elegans.
Journal BMC Genomics
Abstract BACKGROUND:Small RNA-guided transcriptional silencing (nuclear RNAi) is fundamental to genome integrity and epigenetic inheritance. Despite recent progress in identifying the capability and genetic requirements for nuclear RNAi in Caenorhabditis elegans, the natural targets and cellular functions of nuclear RNAi remain elusive.
METHODS:To resolve this gap, we coordinately examined the genome-wide profiles of transcription, histone H3 lysine 9 methylation (H3K9me) and endogenous siRNAs of a germline nuclear Argonaute (hrde-1/wago-9) mutant and identified regions on which transcription activity is markedly increased and/or H3K9me level is markedly decreased relative to wild type animals.
RESULTS:Our data revealed a distinct set of native targets of germline nuclear RNAi, with the H3K9me response exhibiting both overlapping and non-overlapping distribution with the transcriptional silencing response. Interestingly LTR retrotransposons, but not DNA transposons, are highly enriched in the targets of germline nuclear RNAi. The genomic distribution of the native targets is highly constrained, with >99% of the identified targets present in five autosomes but not in the sex chromosome. By contrast, HRDE-1-associated small RNAs correspond to all chromosomes. In addition, we found that the piRNA pathway is not required for germline nuclear RNAi activity on native targets.
CONCLUSION:Germline nuclear RNAi in C. elegans is required to silence retrotransposons but not DNA transposon. Transcriptional silencing and H3K9me can occur independently of each other on the native targets of nuclear RNAi in C. elegans. Our results rule out a simple model in which nuclear Argonaute protein-associated-small RNAs are sufficient to trigger germline nuclear RNAi responses. In addition, the piRNA pathway and germline nuclear RNAi are specialized to target different types of foreign genetic elements for genome surveillance in C. elegans.
Volume 15
Pages 1157
Published 2014-12-22
DOI 10.1186/1471-2164-15-1157
PII 1471-2164-15-1157
PMID 25534009
PMC PMC4367959
MeSH Animals Argonaute Proteins / deficiency Argonaute Proteins / genetics Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Chromatin / genetics Gene Expression Profiling Histones / chemistry* Histones / metabolism* Lysine / metabolism Methylation RNA Interference* RNA Polymerase II / metabolism RNA, Small Interfering / genetics* Retroelements / genetics Transcription, Genetic / genetics*
IF 3.73
Times Cited 8
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
Resource
C.elegans tm1200