| RRC ID |
4587
|
| Author |
Omasa T, Kim K, Hiramatsu S, Katakura Y, Kishimoto M, Enosawa S, Ohtake H.
|
| Title |
Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system.
|
| Journal |
Biotechnol Prog
|
| Abstract |
Focusing on drug metabolism in liver, we constructed and evaluated a drug-metabolizing bioartificial liver (BAL) support system. In a previous study, we constructed ammonia-metabolizing CHO and hepatoma-derived HepG2 cell lines by recombination of the glutamine synthetase (GS) gene. For further mimicking of liver metabolism, the human hepatoma-derived cell line HepG2 was transformed by the pBudCE-GS-CYP3A4 vector, which contains GS and drug-metabolizing CYP 3A4 genes. The constructed GS-3A4-HepG2 cell line showed 3A4 activity higher than that of human primary hepatocytes. The drug-metabolizing activity of BAL (BAL clearance) was evaluated using this cell line. The estimated clearance was higher than that of the human hepatocyte system.
|
| Volume |
21(1)
|
| Pages |
161-7
|
| Published |
2005-1-1
|
| DOI |
10.1021/bp049757a
|
| PMID |
15903254
|
| MeSH |
Ammonia / metabolism
Animals
Bioreactors
CHO Cells
Cell Culture Techniques / methods
Cell Line, Tumor
Cells, Cultured
Cricetinae
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System / genetics
Cytochrome P-450 Enzyme System / metabolism*
Dimercaprol / metabolism*
Dimercaprol / pharmacokinetics
Glutamate-Ammonia Ligase / genetics
Glutamate-Ammonia Ligase / metabolism*
Humans
Liver, Artificial*
Metabolic Clearance Rate
Recombinant Proteins / metabolism
Transfection
|
| IF |
2.334
|
| Times Cited |
9
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
CHO-K1(RCB0285)
Hep G2(RCB0459)
GS-HepG2(RCB1681) |
