RRC ID 45898
Author Yücel D, Hoe M, Llamosas E, Kant S, Jamieson C, Young PA, Crossley M, Nicholas HR.
Title SUMV-1 antagonizes the activity of synthetic multivulva genes in Caenorhabditis elegans.
Journal Dev Biol
Abstract Chromatin regulators contribute to the developmental control of gene expression. In the nematode Caenorhabditis elegans, the roles of chromatin regulation in development have been explored in several contexts, including vulval differentiation. The synthetic multivulva (synMuv) genes are regulators of vulval development in C. elegans and the proteins encoded by these genes include components of several histone modification and chromatin remodelling complexes. By inhibiting ectopic expression of the epidermal growth factor (LIN-3) in the nematode hypodermis, the synMuv genes prevent inappropriate vulval induction. In a forward genetic screen for modifiers of the expression of a hypodermal reporter gene, we identified a mutation that results in increased expression of the reporter. This mutation also suppresses ectopic vulval induction in synMuv mutants and we have consequently named the affected gene suppressor of synthetic multivulva-1 (sumv-1). We show that SUMV-1 is required in the hypodermis for the synMuv phenotype and that loss of sumv-1 function suppresses ectopic expression of lin-3 in synMuv mutant animals. In yeast two-hybrid assays SUMV-1 physically interacts with SUMV-2, and reduction of sumv-2 function also suppresses the synMuv phenotype. We identified similarities between SUMV-1 and SUMV-2 and mammalian proteins KAT8 NSL2 and KAT8 NSL3, respectively, which are components of the KAT8/MOF histone acetyltransferase complex. Reduction of function of mys-2, which encodes the enzymatic component of the KAT8/MOF complex, also suppresses the synMuv phenotype, and MYS-2 physically interacts with SUMV-2 in yeast two-hybrid assays. Together these observations suggest that SUMV-1 and SUMV-2 may function together with MYS-2 in a nematode KAT8/MOF-like complex to antagonise the activity of the synMuv genes.
Volume 392(2)
Pages 266-82
Published 2014-8-15
DOI 10.1016/j.ydbio.2014.05.018
PII S0012-1606(14)00274-7
PMID 24882710
MeSH Animals Base Sequence Blotting, Western Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism DNA Primers / genetics DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Epidermal Growth Factor / antagonists & inhibitors Female Gene Expression Regulation, Developmental / genetics* Histone Acetyltransferases / metabolism In Situ Hybridization, Fluorescence Molecular Sequence Data Polymorphism, Single Nucleotide / genetics RNA Interference Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Two-Hybrid System Techniques Vulva / embryology*
IF 2.896
Times Cited 2
C.elegans tm5512