| RRC ID |
46008
|
| Author |
Al Rawi S, Louvet-Vallée S, Djeddi A, Sachse M, Culetto E, Hajjar C, Boyd L, Legouis R, Galy V.
|
| Title |
Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission.
|
| Journal |
Science
|
| Abstract |
In sexual reproduction of most animals, the spermatozoon provides DNA and centrioles, together with some cytoplasm and organelles, to the oocyte that is being fertilized. Paternal mitochondria and their genomes are generally eliminated in the embryo by an unknown degradation mechanism. We show that, upon fertilization, a Caenorhabditis elegans spermatozoon triggers the recruitment of autophagosomes within minutes and subsequent paternal mitochondria degradation. Whereas the nematode-specific sperm membranous organelles are ubiquitinated before autophagosome formation, the mitochondria are not. The degradation of both paternal structures and mitochondrial DNA requires an LC3-dependent autophagy. Analysis of fertilized mouse embryos shows the localization of autophagy markers, which suggests that this autophagy event is evolutionarily conserved to prevent both the transmission of paternal mitochondrial DNA to the offspring and the establishment of heteroplasmy.
|
| Volume |
334(6059)
|
| Pages |
1144-7
|
| Published |
2011-11-25
|
| DOI |
10.1126/science.1211878
|
| PII |
science.1211878
|
| PMID |
22033522
|
| MeSH |
Animals
Autophagy*
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / physiology
Caenorhabditis elegans Proteins / analysis
DNA, Mitochondrial / analysis
DNA, Mitochondrial / genetics*
DNA, Mitochondrial / metabolism
Embryo, Nonmammalian / physiology*
Embryonic Development
Female
Fertilization
Hermaphroditic Organisms
Lysosomes / metabolism
Male
Mice
Mitochondria / metabolism*
Oocytes / physiology
Organelles / metabolism
Phagosomes / metabolism
Spermatozoa / chemistry
Spermatozoa / physiology
Spermatozoa / ultrastructure*
Ubiquitination
|
| IF |
41.846
|
| Times Cited |
248
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm3489 |
