論文 - 詳細
| RRC ID | 46016 |
|---|---|
| 著者 | Arnadóttir J, O'Hagan R, Chen Y, Goodman MB, Chalfie M. |
| タイトル | The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons. |
| ジャーナル | J Neurosci |
| Abstract |
Gentle touch sensation in Caenorhabditis elegans is mediated by the MEC-4/MEC-10 channel complex, which is expressed exclusively in six touch receptor neurons (TRNs). The complex contains two pore-forming subunits, MEC-4 and MEC-10, as well as the accessory subunits MEC-2, MEC-6, and UNC-24. MEC-4 is essential for channel function, but beyond its role as a pore-forming subunit, the functional contribution of MEC-10 to the channel complex and to touch sensation is unclear. We addressed this question using behavioral assays, in vivo electrophysiological recordings from TRNs, and heterologous expression of mutant MEC-10 isoforms. Animals with a deletion in mec-10 showed only a partial loss of touch sensitivity and a modest decrease in the size of the mechanoreceptor current (MRC). In contrast, five previously identified mec-10 alleles acted as recessive gain-of-function alleles that resulted in complete touch insensitivity. Each of these alleles produced a substantial decrease in MRC size and a shift in the reversal potential in vivo. The latter finding indicates that these mec-10 mutations alter the ionic selectivity of the transduction channel in vivo. All mec-10 mutant animals had properly localized channel complexes, indicating that the loss of MRCs was not attributable to a dramatic mislocalization of transduction channels. Finally, electrophysiological examination of heterologously expressed complexes suggests that mutant MEC-10 proteins may affect channel current via MEC-2. |
| 巻・号 | 31(35) |
| ページ | 12695-704 |
| 公開日 | 2011-8-31 |
| DOI | 10.1523/JNEUROSCI.4580-10.2011 |
| PII | 31/35/12695 |
| PMID | 21880930 |
| PMC | PMC3172708 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation / genetics Immunoprecipitation / methods In Vitro Techniques Mechanotransduction, Cellular / genetics Mechanotransduction, Cellular / physiology* Membrane Potentials / genetics Membrane Potentials / physiology Membrane Proteins / deficiency Membrane Proteins / genetics Membrane Proteins / metabolism* Microinjections / methods Mutation, Missense / genetics Oocytes Physical Stimulation / methods Point Mutation / genetics RNA Interference / physiology Sensory Receptor Cells / metabolism* Sodium Channels / genetics Sodium Channels / metabolism Touch / genetics* |
| IF | 5.674 |
| 引用数 | 42 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1552 |