論文 - 詳細
| RRC ID | 46026 |
|---|---|
| 著者 | Bernadskaya YY, Patel FB, Hsu HT, Soto MC. |
| タイトル | Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins. |
| ジャーナル | Mol Biol Cell |
| Abstract |
It has been proposed that Arp2/3, which promotes nucleation of branched actin, is needed for epithelial junction initiation but is less important as junctions mature. We focus here on how Arp2/3 contributes to the Caenorhabditis elegans intestinal epithelium and find important roles for Arp2/3 in the maturation and maintenance of junctions in embryos and adults. Electron microscope studies show that embryos depleted of Arp2/3 form apical actin-rich microvilli and electron-dense apical junctions. However, whereas apical/basal polarity initiates, apical maturation is defective, including decreased apical F-actin enrichment, aberrant lumen morphology, and reduced accumulation of some apical junctional proteins, including DLG-1. Depletion of Arp2/3 in adult animals leads to similar intestinal defects. The DLG-1/AJM-1 apical junction proteins, and the ezrin-radixin-moesin homologue ERM-1, a protein that connects F-actin to membranes, are required along with Arp2/3 for apical F-actin enrichment in embryos, whereas cadherin junction proteins are not. Arp2/3 affects the subcellular distribution of DLG-1 and ERM-1. Loss of Arp2/3 shifts both ERM-1 and DLG-1 from pellet fractions to supernatant fractions, suggesting a role for Arp2/3 in the distribution of membrane-associated proteins. Thus, Arp2/3 is required as junctions mature to maintain apical proteins associated with the correct membranes. |
| 巻・号 | 22(16) |
| ページ | 2886-99 |
| 公開日 | 2011-8-15 |
| DOI | 10.1091/mbc.E10-10-0862 |
| PII | mbc.E10-10-0862 |
| PMID | 21697505 |
| PMC | PMC3154884 |
| MeSH | Actin-Related Protein 2-3 Complex / genetics Actin-Related Protein 2-3 Complex / metabolism* Actins / metabolism Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Membrane / metabolism* Cytoskeletal Proteins / metabolism Embryonic Development Intercellular Junctions / metabolism* Intestinal Mucosa / embryology Intestinal Mucosa / metabolism Intestinal Mucosa / ultrastructure Intestines / cytology* Larva / cytology Larva / metabolism Membrane Proteins / metabolism Microscopy, Fluorescence Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Phalloidine / metabolism Phenotype Protein Transport RNA Interference Subcellular Fractions / metabolism |
| IF | 3.791 |
| 引用数 | 26 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm677 |