| RRC ID |
46040
|
| Author |
Cao P, Sun W, Kramp K, Zheng M, Salom D, Jastrzebska B, Jin H, Palczewski K, Feng Z.
|
| Title |
Light-sensitive coupling of rhodopsin and melanopsin to G(i/o) and G(q) signal transduction in Caenorhabditis elegans.
|
| Journal |
FASEB J
|
| Abstract |
Activation of G-protein-coupled receptors (GPCRs) initiates signal transduction cascades that affect many physiological responses. The worm Caenorhabditis elegans expresses >1000 of these receptors along with their cognate heterotrimeric G proteins. Here, we report properties of 9-cis-retinal regenerated bovine opsin [(b)isoRho] and human melanopsin [(h)Mo], two light-activated, heterologously expressed GPCRs in the nervous system of C. elegans with various genetically engineered alterations. Profound transient photoactivation of G(i/o) signaling by (b)isoRho led to a sudden and transient loss of worm motility dependent on cyclic adenosine monophosphate, whereas transient photoactivation of G(q) signaling by (h)Mo enhanced worm locomotion dependent on phospholipase Cβ. These transgenic C. elegans models provide a unique way to study the consequences of G(i/o) and G(q) signaling in vivo with temporal and spatial precision and, by analogy, their relationship to human neuromotor function.
|
| Volume |
26(2)
|
| Pages |
480-91
|
| Published |
2012-2-1
|
| DOI |
10.1096/fj.11-197798
|
| PII |
fj.11-197798
|
| PMID |
22090313
|
| PMC |
PMC3290438
|
| MeSH |
3',5'-Cyclic-AMP Phosphodiesterases / metabolism
Animals
Animals, Genetically Modified
Base Sequence
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans / physiology
Caenorhabditis elegans / radiation effects
Caenorhabditis elegans Proteins / metabolism*
Cattle
DNA Primers / genetics
Diterpenes
GTP-Binding Protein alpha Subunits, Gi-Go / metabolism*
GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism*
Humans
Light
Models, Biological
Motor Activity / radiation effects
Neurons / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Retinaldehyde / metabolism
Rhodopsin / genetics
Rhodopsin / metabolism*
Rod Opsins / genetics
Rod Opsins / metabolism*
Signal Transduction
|
| IF |
4.966
|
| Times Cited |
19
|
|
WOS Category
|
BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm3098 |
