論文 - 詳細
| RRC ID | 46046 |
|---|---|
| 著者 | Checchi PM, Engebrecht J. |
| タイトル | Caenorhabditis elegans histone methyltransferase MET-2 shields the male X chromosome from checkpoint machinery and mediates meiotic sex chromosome inactivation. |
| ジャーナル | PLoS Genet |
| Abstract |
Meiosis is a specialized form of cellular division that results in the precise halving of the genome to produce gametes for sexual reproduction. Checkpoints function during meiosis to detect errors and subsequently to activate a signaling cascade that prevents the formation of aneuploid gametes. Indeed, asynapsis of a homologous chromosome pair elicits a checkpoint response that can in turn trigger germline apoptosis. In a heterogametic germ line, however, sex chromosomes proceed through meiosis with unsynapsed regions and are not recognized by checkpoint machinery. We conducted a directed RNAi screen in Caenorhabditis elegans to identify regulatory factors that prevent recognition of heteromorphic sex chromosomes as unpaired and uncovered a role for the SET domain histone H3 lysine 9 histone methyltransferase (HMTase) MET-2 and two additional HMTases in shielding the male X from checkpoint machinery. We found that MET-2 also mediates the transcriptional silencing program of meiotic sex chromosome inactivation (MSCI) but not meiotic silencing of unsynapsed chromatin (MSUC), suggesting that these processes are distinct. Further, MSCI and checkpoint shielding can be uncoupled, as double-strand breaks targeted to an unpaired, transcriptionally silenced extra-chromosomal array induce checkpoint activation in germ lines depleted for met-2. In summary, our data uncover a mechanism by which repressive chromatin architecture enables checkpoint proteins to distinguish between the partnerless male X chromosome and asynapsed chromosomes thereby shielding the lone X from inappropriate activation of an apoptotic program. |
| 巻・号 | 7(9) |
| ページ | e1002267 |
| 公開日 | 2011-9-1 |
| DOI | 10.1371/journal.pgen.1002267 |
| PII | PGENETICS-D-11-00601 |
| PMID | 21909284 |
| PMC | PMC3164706 |
| MeSH | Animals Apoptosis Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Chromatin / genetics Chromatin / metabolism Gene Silencing Genes, cdc Germ Cells / cytology Germ Cells / metabolism Histone-Lysine N-Methyltransferase / genetics* Histone-Lysine N-Methyltransferase / metabolism* Histones / genetics* Histones / metabolism* Lysine / genetics Lysine / metabolism Male Meiosis / genetics* Transcription, Genetic X Chromosome / genetics X Chromosome Inactivation / genetics* |
| IF | 5.175 |
| 引用数 | 18 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1276 tm1458 tm1813 tm574 |