論文 - 詳細
| RRC ID | 46071 |
|---|---|
| 著者 | Maekawa M, Terasaka S, Mochizuki Y, Kawai K, Ikeda Y, Araki N, Skolnik EY, Taguchi T, Arai H. |
| タイトル | Sequential breakdown of 3-phosphorylated phosphoinositides is essential for the completion of macropinocytosis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and solutes are internalized into cells. Macropinocytosis starts with the formation of membrane ruffles at the plasma membrane and ends with their closure. The transient and sequential emergence of phosphoinositides PI(3,4,5)P3 and PI(3,4)P2 in the membrane ruffles is essential for macropinocytosis. By making use of information in the Caenorhabditis elegans mutants defective in fluid-phase endocytosis, we found that mammalian phosphoinositide phosphatase MTMR6 that dephosphorylates PI(3)P to PI, and its binding partner MTMR9, are required for macropinocytosis. INPP4B, which dephosphorylates PI(3,4)P2 to PI(3)P, was also found to be essential for macropinocytosis. These phosphatases operate after the formation of membrane ruffles to complete macropinocytosis. Finally, we showed that KCa3.1, a Ca(2+)-activated K(+) channel that is activated by PI(3)P, is required for macropinocytosis. We propose that the sequential breakdown of PI(3,4,5)P3 → PI(3,4)P2 → PI(3)P → PI controls macropinocytosis through specific effectors of the intermediate phosphoinositides. |
| 巻・号 | 111(11) |
| ページ | E978-87 |
| 公開日 | 2014-3-18 |
| DOI | 10.1073/pnas.1311029111 |
| PII | 1311029111 |
| PMID | 24591580 |
| PMC | PMC3964036 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Cell Line DNA Primers / genetics Humans Microscopy, Electron, Scanning Microscopy, Fluorescence Phosphatidylinositol Phosphates / metabolism* Phosphoric Monoester Hydrolases / metabolism* Phosphorylation Pinocytosis / physiology* Protein Tyrosine Phosphatases, Non-Receptor / metabolism* RNA Interference RNA, Small Interfering / genetics Reverse Transcriptase Polymerase Chain Reaction |
| IF | 9.412 |
| 引用数 | 40 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1125 tm3733 tm2036 tm246 |