RRC ID 46092
Author Murayama T, Takayama J, Fujiwara M, Maruyama IN.
Title Environmental alkalinity sensing mediated by the transmembrane guanylyl cyclase GCY-14 in C. elegans.
Journal Curr Biol
Abstract Survival requires that living organisms continuously monitor environmental and tissue pH. Animals sense acidic pH using ion channels and G-protein-coupled receptors (GPCRs), but monitoring of alkaline pH is not well understood. We report here that in the nematode Caenorhabditis elegans, a transmembrane receptor-type guanylyl cyclase (RGC), GCY-14, of the ASEL gustatory neuron, plays an essential role in the sensing of extracellular alkalinity. Activation of GCY-14 opens a cGMP-gated cation channel encoded by tax-2 and tax-4, resulting in Ca(2+) entry into ASEL. Ectopic expression of GCY-14 in other neurons indicates that it accounts for the alkalinity sensing capability. Domain-swapping and site-directed mutagenesis of GCY-14 reveal that GCY-14 functions as a homodimer, in which histidine of the extracellular domains plays a crucial role in alkalinity detection. These results argue that in addition to ion channels and GPCRs, RGCs also play a role in pH sensation in neurons.
Volume 23(11)
Pages 1007-12
Published 2013-6-3
DOI 10.1016/j.cub.2013.04.052
PII S0960-9822(13)00493-4
PMID 23664973
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Calcium Signaling* Cyclic GMP / metabolism* Guanylate Cyclase / genetics* Guanylate Cyclase / metabolism Hydrogen-Ion Concentration Ion Channels / metabolism Molecular Sequence Data Neurons / metabolism Neurons / physiology
IF 9.601
Times Cited 18
C.elegans tm3098 tm1449 tm901 tm2669 tm1653 tm897 tm2364