論文 - 詳細
| RRC ID | 46107 |
|---|---|
| 著者 | Hao L, Thein M, Brust-Mascher I, Civelekoglu-Scholey G, Lu Y, Acar S, Prevo B, Shaham S, Scholey JM. |
| タイトル | Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Sensory cilia are assembled and maintained by kinesin-2-dependent intraflagellar transport (IFT). We investigated whether two Caenorhabditis elegans α- and β-tubulin isotypes, identified through mutants that lack their cilium distal segments, are delivered to their assembly sites by IFT. Mutations in conserved residues in both tubulins destabilize distal singlet microtubules. One isotype, TBB-4, assembles into microtubules at the tips of the axoneme core and distal segments, where the microtubule tip tracker EB1 is found, and localizes all along the cilium, whereas the other, TBA-5, concentrates in distal singlets. IFT assays, fluorescence recovery after photobleaching analysis and modelling indicate that the continual transport of sub-stoichiometric numbers of these tubulin subunits by the IFT machinery can maintain sensory cilia at their steady-state length. |
| 巻・号 | 13(7) |
| ページ | 790-8 |
| 公開日 | 2011-6-5 |
| DOI | 10.1038/ncb2268 |
| PII | ncb2268 |
| PMID | 21642982 |
| PMC | PMC3129367 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism Flagella / metabolism* Fluorescence Recovery After Photobleaching Mechanotransduction, Cellular* Microscopy, Fluorescence Microtubule-Associated Proteins / metabolism Microtubules / genetics Microtubules / metabolism* Models, Biological Mutation Protein Transport Sensation* Time Factors Tubulin / genetics Tubulin / metabolism* |
| IF | 20.042 |
| 引用数 | 96 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 線虫 | tm324 tm4200 tm2355 tm2394 |