RRC ID 46107
著者 Hao L, Thein M, Brust-Mascher I, Civelekoglu-Scholey G, Lu Y, Acar S, Prevo B, Shaham S, Scholey JM.
タイトル Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments.
ジャーナル Nat Cell Biol
Abstract Sensory cilia are assembled and maintained by kinesin-2-dependent intraflagellar transport (IFT). We investigated whether two Caenorhabditis elegans α- and β-tubulin isotypes, identified through mutants that lack their cilium distal segments, are delivered to their assembly sites by IFT. Mutations in conserved residues in both tubulins destabilize distal singlet microtubules. One isotype, TBB-4, assembles into microtubules at the tips of the axoneme core and distal segments, where the microtubule tip tracker EB1 is found, and localizes all along the cilium, whereas the other, TBA-5, concentrates in distal singlets. IFT assays, fluorescence recovery after photobleaching analysis and modelling indicate that the continual transport of sub-stoichiometric numbers of these tubulin subunits by the IFT machinery can maintain sensory cilia at their steady-state length.
巻・号 13(7)
ページ 790-8
公開日 2011-6-5
DOI 10.1038/ncb2268
PII ncb2268
PMID 21642982
PMC PMC3129367
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism Flagella / metabolism* Fluorescence Recovery After Photobleaching Mechanotransduction, Cellular* Microscopy, Fluorescence Microtubule-Associated Proteins / metabolism Microtubules / genetics Microtubules / metabolism* Models, Biological Mutation Protein Transport Sensation* Time Factors Tubulin / genetics Tubulin / metabolism*
IF 20.042
引用数 96
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm324 tm4200 tm2355 tm2394