Reference - Detail
RRC ID | 46121 |
---|---|
Author | Hu Z, Pym EC, Babu K, Vashlishan Murray AB, Kaplan JM. |
Title | A neuropeptide-mediated stretch response links muscle contraction to changes in neurotransmitter release. |
Journal | Neuron |
Abstract |
Although Caenorhabditis elegans has been utilized extensively to study synapse formation and function, relatively little is known about synaptic plasticity in C. elegans. We show that a brief treatment with the cholinesterase inhibitor aldicarb induces a form of presynaptic potentiation whereby ACh release at neuromuscular junctions (NMJs) is doubled. Aldicarb-induced potentiation was eliminated by mutations that block processing of proneuropeptides, by mutations inactivating a single proneuropeptide (NLP-12), and by those inactivating an NLP-12 receptor (CKR-2). NLP-12 expression is limited to a single stretch-activated neuron, DVA. Analysis of a YFP-tagged NLP-12 suggests that aldicarb stimulates DVA secretion of NLP-12. Mutations disrupting the DVA mechanoreceptor (TRP-4) decreased aldicarb-induced NLP-12 secretion and blocked aldicarb-induced synaptic potentiation. Mutants lacking NLP-12 or CKR-2 have decreased locomotion rates. Collectively, these results suggest that NLP-12 mediates a mechanosensory feedback loop that couples muscle contraction to changes in presynaptic release, thereby providing a mechanism for proprioceptive control of locomotion. |
Volume | 71(1) |
Pages | 92-102 |
Published | 2011-7-14 |
DOI | 10.1016/j.neuron.2011.04.021 |
PII | S0896-6273(11)00377-1 |
PMID | 21745640 |
PMC | PMC3134788 |
MeSH | Acetylcholine / metabolism Aldicarb / pharmacology Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cholinesterase Inhibitors / pharmacology Inhibitory Postsynaptic Potentials / genetics Inhibitory Postsynaptic Potentials / physiology Locomotion / physiology Mechanoreceptors / metabolism* Mechanoreceptors / physiology Muscle Contraction / physiology* Mutation Neuronal Plasticity / genetics Neuronal Plasticity / physiology* Neuropeptides / genetics Neuropeptides / metabolism* Proprotein Convertase 2 / genetics Receptors, CCR2 / genetics Synaptic Transmission / drug effects Synaptic Transmission / genetics Synaptic Transmission / physiology* TRPC Cation Channels / genetics |
IF | 14.415 |
Times Cited | 43 |
WOS Category | NEUROSCIENCES |
Resource | |
C.elegans | tm3082 |