RRC ID 46125
Author Patel FB, Soto MC.
Title WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia.
Journal Dev. Biol.
Abstract Cells can use the force of actin polymerization to drive intracellular transport, but the role of actin in endocytosis is not clear. Studies in single-celled yeast demonstrate the essential role of the branched actin nucleator, Arp2/3, and its activating nucleation promoting factors (NPFs) in the process of invagination from the cell surface through endocytosis. However, some mammalian studies have disputed the need for F-actin and Arp2/3 in Clathrin-Mediated Endocytosis (CME) in multicellular organisms. We investigate the role of Arp2/3 during endocytosis in Caenorhabditis elegans, a multicellular organism with polarized epithelia. Arp2/3 and its NPF, WAVE/SCAR, are essential for C. elegans embryonic morphogenesis. We show that WAVE/SCAR and Arp2/3 regulate endocytosis and early endosome morphology in diverse tissues of C. elegans. Depletion of WAVE/SCAR or Arp2/3, but not of the NPF Wasp, severely disrupts the distribution of molecules proposed to be internalized via CME, and alters the subcellular enrichment of the early endosome regulator RAB-5. Loss of WAVE/SCAR or of the GEFs that regulate RAB-5 results in similar defects in endocytosis in the intestine and coelomocyte cells. This study in a multicellular organism supports an essential role for branched actin regulators in endocytosis, and identifies WAVE/SCAR as a key NPF that promotes Arp2/3 endocytic function in C. elegans.
Volume 377(2)
Pages 319-32
Published 2013-5-15
DOI 10.1016/j.ydbio.2013.03.012
PII S0012-1606(13)00141-3
PMID 23510716
PMC PMC3700809
MeSH Actin-Related Protein 2-3 Complex / metabolism* Actins / metabolism Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* Endocytosis / physiology* Endosomes Green Fluorescent Proteins Models, Biological Morphogenesis / physiology* RNA Interference Vesicular Transport Proteins / metabolism
IF 2.936
Times Cited 4
WOS Category DEVELOPMENTAL BIOLOGY
Resource
C.elegans tm597