論文 - 詳細
| RRC ID | 46136 |
|---|---|
| 著者 | Kang J, Bai Z, Zegarek MH, Grant BD, Lee J. |
| タイトル | Essential roles of snap-29 in C. elegans. |
| ジャーナル | Dev Biol |
| Abstract |
SNARE domain proteins are key molecules mediating intracellular fusion events. SNAP25 family proteins are unique target-SNAREs possessing two SNARE domains. Here we report the genetic, molecular, and cell biological characterization of C. elegans SNAP-29. We found that snap-29 is an essential gene required throughout the life-cycle. Depletion of snap-29 by RNAi in adults results in sterility associated with endomitotic oocytes and pre-meiotic maturation of the oocytes. Many of the embryos that are produced are multinucleated, indicating a defect in embryonic cytokinesis. A profound defect in secretion by oocytes and early embryos in animals lacking SNAP-29 appears to be the underlying defect connecting these phenotypes. Further analysis revealed defects in basolateral and apical secretion by intestinal epithelial cells in animals lacking SNAP-29, indicating a broad requirement for this protein in the secretory pathway. A SNAP-29-GFP fusion protein was enriched on recycling endosomes, and loss of SNAP-29 disrupted recycling endosome morphology. Taken together these results suggest a requirement for SNAP-29 in the fusion of post-Golgi vesicles with the recycling endosome for cargo to reach the cell surface. |
| 巻・号 | 355(1) |
| ページ | 77-88 |
| 公開日 | 2011-7-1 |
| DOI | 10.1016/j.ydbio.2011.04.013 |
| PII | S0012-1606(11)00245-4 |
| PMID | 21545795 |
| PMC | PMC3118655 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Endosomes / physiology* Female Golgi Apparatus / physiology* Infertility / genetics* Male Oocytes / metabolism Oocytes / physiology Q-SNARE Proteins / genetics Q-SNARE Proteins / metabolism Q-SNARE Proteins / physiology* RNA Interference SNARE Proteins / genetics SNARE Proteins / metabolism SNARE Proteins / physiology* |
| IF | 2.896 |
| 引用数 | 16 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2060 |