RRC ID 4615
著者 Sakudoh T, Sezutsu H, Nakashima T, Kobayashi I, Fujimoto H, Uchino K, Banno Y, Iwano H, Maekawa H, Tamura T, Kataoka H, Tsuchida K.
タイトル Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene.
ジャーナル Proc Natl Acad Sci U S A
Abstract Mechanisms for the uptake and transport of carotenoids, essential nutrients for humans, are not well understood in any animal system. The Y (Yellow blood) gene, a critical cocoon color determinant in the silkworm Bombyx mori, controls the uptake of carotenoids into the intestinal mucosa and the silk gland. Here we provide evidence that the Y gene corresponds to the intracellular carotenoid-binding protein (CBP) gene. In the Y recessive strain, the absence of an exon, likely due to an incorrect mRNA splicing caused by a transposon-associated genomic deletion, generates a nonfunctional CBP mRNA, resulting in colorless hemolymph and white cocoons. Enhancement of carotenoid uptake and coloration of the white cocoon was achieved by germ-line transformation with the CBP gene. This study demonstrates the existence of a genetically facilitated intracellular process beyond passive diffusion for carotenoid uptake in the animal phyla, and paves the way for modulating silk color and lipid content through genetic engineering.
巻・号 104(21)
ページ 8941-6
公開日 2007-5-22
DOI 10.1073/pnas.0702860104
PII 0702860104
解説 Y(黄血)の原因遺伝子はCBPである。CBPはカロテノイド結合タンパク質をコードする。FL50系統(+Y/+Y)では、CBPのエクソン2の3'側169bpが欠失している。
PMID 17496138
PMC PMC1885607
MeSH Alleles Animals Animals, Genetically Modified Bombyx / genetics Bombyx / metabolism Carotenoids / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Color Gene Expression Regulation Genome, Insect / genetics Insect Proteins / genetics Insect Proteins / metabolism* Molecular Sequence Data Phenotype Silk / metabolism*
IF 9.412
引用数 77
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ FL50