論文 - 詳細
| RRC ID | 46173 |
|---|---|
| 著者 | Riedel CG, Dowen RH, Lourenco GF, Kirienko NV, Heimbucher T, West JA, Bowman SK, Kingston RE, Dillin A, Asara JM, Ruvkun G. |
| タイトル | DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Organisms are constantly challenged by stresses and privations and require adaptive responses for their survival. The forkhead box O (FOXO) transcription factor DAF-16 (hereafter referred to as DAF-16/FOXO) is a central nexus in these responses, but despite its importance little is known about how it regulates its target genes. Proteomic identification of DAF-16/FOXO-binding partners in Caenorhabditis elegans and their subsequent functional evaluation by RNA interference revealed several candidate DAF-16/FOXO cofactors, most notably the chromatin remodeller SWI/SNF. DAF-16/FOXO and SWI/SNF form a complex and globally co-localize at DAF-16/FOXO target promoters. We show that specifically for gene activation, DAF-16/FOXO depends on SWI/SNF, facilitating SWI/SNF recruitment to target promoters, to activate transcription by presumed remodelling of local chromatin. For the animal, this translates into an essential role for SWI/SNF in DAF-16/FOXO-mediated processes, in particular dauer formation, stress resistance and the promotion of longevity. Thus, we give insight into the mechanisms of DAF-16/FOXO-mediated transcriptional regulation and establish a critical link between ATP-dependent chromatin remodelling and lifespan regulation. |
| 巻・号 | 15(5) |
| ページ | 491-501 |
| 公開日 | 2013-5-1 |
| DOI | 10.1038/ncb2720 |
| PII | ncb2720 |
| PMID | 23604319 |
| PMC | PMC3748955 |
| MeSH | Adaptation, Physiological Animals Binding Sites Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromatin Assembly and Disassembly* Chromatography, Gel Escherichia coli / genetics Escherichia coli / metabolism Forkhead Transcription Factors Gene Expression Regulation Kaplan-Meier Estimate Longevity* Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Nucleotide Motifs Oxidative Stress Promoter Regions, Genetic Protein Binding RNA Interference Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation |
| IF | 20.042 |
| 引用数 | 105 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3647 |