| RRC ID |
46179
|
| 著者 |
Ruf V, Holzem C, Peyman T, Walz G, Blackwell TK, Neumann-Haefelin E.
|
| タイトル |
TORC2 signaling antagonizes SKN-1 to induce C. elegans mesendodermal embryonic development.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The evolutionarily conserved target of rapamycin (TOR) kinase controls fundamental metabolic processes to support cell and tissue growth. TOR functions within the context of two distinct complexes, TORC1 and TORC2. TORC2, with its specific component Rictor, has been recently implicated in aging and regulation of growth and metabolism. Here, we identify rict-1/Rictor as a regulator of embryonic development in C. elegans. The transcription factor skn-1 establishes development of the mesendoderm in embryos, and is required for cellular homeostasis and longevity in adults. Loss of maternal skn-1 function leads to mis-specification of the mesendodermal precursor and failure to form intestine and pharynx. We found that genetic inactivation of rict-1 suppressed skn-1-associated lethality by restoring mesendodermal specification in skn-1 deficient embryos. Inactivation of other TORC2 but not TORC1 components also partially rescued skn-1 embryonic lethality. The SGK-1 kinase mediated these functions downstream of rict-1/TORC2, as a sgk-1 gain-of-function mutant suppressed the rict-1 mutant phenotype. These data indicate that TORC2 and SGK-1 antagonize SKN-1 during embryonic development.
|
| 巻・号 |
384(2)
|
| ページ |
214-27
|
| 公開日 |
2013-12-15
|
| DOI |
10.1016/j.ydbio.2013.08.011
|
| PII |
S0012-1606(13)00436-3
|
| PMID |
23973804
|
| PMC |
PMC3877771
|
| MeSH |
Animals
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / antagonists & inhibitors*
DNA-Binding Proteins / antagonists & inhibitors*
Embryo, Nonmammalian / cytology*
Endoderm / embryology*
Genes, Lethal
Mechanistic Target of Rapamycin Complex 2
Mesoderm / embryology*
Multiprotein Complexes / metabolism*
Signal Transduction*
TOR Serine-Threonine Kinases / metabolism*
Transcription Factors / antagonists & inhibitors*
|
| IF |
2.896
|
| 引用数 |
10
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm3411
tm2386 |
