| RRC ID |
46231
|
| 著者 |
Wang Z, Hou Y, Guo X, van der Voet M, Boxem M, Dixon JE, Chisholm AD, Jin Y.
|
| タイトル |
The EBAX-type Cullin-RING E3 ligase and Hsp90 guard the protein quality of the SAX-3/Robo receptor in developing neurons.
|
| ジャーナル |
Neuron
|
| Abstract |
Although protein quality control (PQC) is generally perceived as important for the development of the nervous system, the specific mechanisms of neuronal PQC have remained poorly understood. Here, we report that C. elegans Elongin BC-binding axon regulator (EBAX-1), a conserved BC-box protein, regulates axon guidance through PQC of the SAX-3/Robo receptor. EBAX-1 buffers guidance errors against temperature variations. As a substrate-recognition subunit in the Elongin BC-containing Cullin-RING ubiquitin ligase (CRL), EBAX-1 also binds to DAF-21, a cytosolic Hsp90 chaperone. The EBAX-type CRL and DAF-21 collaboratively regulate SAX-3-mediated axon pathfinding. Biochemical and imaging assays indicate that EBAX-1 specifically recognizes misfolded SAX-3 and promotes its degradation in vitro and in vivo. Importantly, vertebrate EBAX also shows substrate preference toward aberrant Robo3 implicated in horizontal gaze palsy with progressive scoliosis (HGPPS). Together, our findings demonstrate a triage PQC mechanism mediated by the EBAX-type CRL and DAF-21/Hsp90 that maintains the accuracy of neuronal wiring.
|
| 巻・号 |
79(5)
|
| ページ |
903-16
|
| 公開日 |
2013-9-4
|
| DOI |
10.1016/j.neuron.2013.06.035
|
| PII |
S0896-6273(13)00551-5
|
| PMID |
24012004
|
| PMC |
PMC3779136
|
| MeSH |
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins / physiology*
Cullin Proteins / physiology*
Drosophila
Elongin
HSP90 Heat-Shock Proteins
Mice
Nerve Tissue Proteins / biosynthesis
Nerve Tissue Proteins / metabolism*
Nervous System / embryology*
Nervous System / metabolism
Neurogenesis
Neurons
Receptors, Immunologic / biosynthesis
Receptors, Immunologic / metabolism*
Ubiquitin-Protein Ligases / physiology*
|
| IF |
14.415
|
| 引用数 |
8
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 線虫 |
tm2321 |
