論文 - 詳細
| RRC ID | 46250 |
|---|---|
| 著者 | Zhuang JJ, Banse SA, Hunter CP. |
| タイトル | The nuclear argonaute NRDE-3 contributes to transitive RNAi in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
The Caenorhabditis elegans nuclear RNA interference defective (Nrde) mutants were identified by their inability to silence polycistronic transcripts in enhanced RNAi (Eri) mutant backgrounds. Here, we report additional nrde-3-dependent RNAi phenomena that extend the mechanisms, roles, and functions of nuclear RNAi. We show that nrde-3 mutants are broadly RNAi deficient and that overexpressing NRDE-3 enhances RNAi. Consistent with NRDE-3 being a dose-dependent limiting resource for effective RNAi, we find that NRDE-3 is required for eri-dependent enhanced RNAi phenotypes, although only for a subset of target genes. We then identify pgl-1 as an additional limiting RNAi resource important for eri-dependent silencing of a nonoverlapping subset of target genes, so that an nrde-3; pgl-1; eri-1 triple mutant fails to show enhanced RNAi for any tested gene. These results suggest that nrde-3 and pgl-1 define separate and independent limiting RNAi resource pathways. Limiting RNAi resources are proposed to primarily act via endogenous RNA silencing pathways. Consistent with this, we find that nrde-3 mutants misexpress genes regulated by endogenous siRNAs and incompletely silence repetitive transgene arrays. Finally, we find that nrde-3 contributes to transitive RNAi, whereby amplified silencing triggers act in trans to silence sequence-similar genes. Because nrde-dependent silencing is thought to act in cis to limit the production of primary transcripts, this result reveals an unexpected role for nuclear processes in RNAi silencing. |
| 巻・号 | 194(1) |
| ページ | 117-31 |
| 公開日 | 2013-5-1 |
| DOI | 10.1534/genetics.113.149765 |
| PII | genetics.113.149765 |
| PMID | 23457236 |
| PMC | PMC3632460 |
| MeSH | Alleles Animals Argonaute Proteins / metabolism* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Cell Nucleus / metabolism* Exoribonucleases / metabolism Gene Expression Regulation Mutation / genetics Phenotype RNA Interference* RNA, Double-Stranded / metabolism RNA-Binding Proteins / metabolism* Transgenes / genetics |
| IF | 4.015 |
| 引用数 | 13 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1860 tm1917 tm1624 tm1276 tm572 |