RRC ID 46281
Author Castellano-Pozo M, García-Muse T, Aguilera A.
Title R-loops cause replication impairment and genome instability during meiosis.
Journal EMBO Rep
Abstract R-loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R-loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results.
Volume 13(10)
Pages 923-9
Published 2012-10-1
DOI 10.1038/embor.2012.119
PII embor2012119
PMID 22878416
PMC PMC3463965
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA Damage / genetics DNA Replication / genetics* DNA, Fungal / chemistry DNA, Fungal / metabolism Genomic Instability / genetics* Meiosis / genetics* Nuclear Proteins / genetics Nuclear Proteins / metabolism Ribonuclease H / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism*
IF 7.497
Times Cited 43
C.elegans tm1310