| RRC ID |
46285
|
| Author |
Cha DS, Hollis SE, Datla US, Lee S, Ryu J, Jung HR, Kim E, Kim K, Lee M, Li C, Lee MH.
|
| Title |
Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development.
|
| Journal |
Gene Expr Patterns
|
| Abstract |
DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.
|
| Volume |
12(5-6)
|
| Pages |
189-95
|
| Published |
2012-1-1
|
| DOI |
10.1016/j.gep.2012.03.002
|
| PII |
S1567-133X(12)00026-9
|
| PMID |
22452997
|
| MeSH |
Alternative Splicing
Animals
Caenorhabditis elegans / chemistry
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism
Chromosome Segregation
DNA Topoisomerases, Type I / analysis
DNA Topoisomerases, Type I / metabolism*
Gonads / embryology
Gonads / metabolism
Isoenzymes / analysis
Isoenzymes / metabolism
|
| IF |
0.897
|
| Times Cited |
3
|
|
WOS Category
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| C.elegans |
tm3126 |
